LOX-1在2型糖尿病下肢动脉硬化闭塞症中的表达及其作用

目的:观察2型糖尿病下肢动脉硬化闭塞症中血凝素样氧化低密度脂蛋白受体-1(LOX-1)mRNA和蛋白表达,并探讨其作用机制。方法:对9例2型糖尿病下肢动脉硬化闭塞症患者(糖尿病组)和7例正常肾移植供肾者(对照组)动脉标本行HE染色,应用RT-PCR和Western-blot检测动脉组织匀浆中LOX-1的mRNA和蛋白水平。结果:与对照组相比,糖尿病组LOX-1mRNA和蛋白水平表达明显升高(P0.05)。结论:2型糖尿病患者可能通过上调LOX-1表达而更容易合并下肢动脉硬化闭塞症,LOX-1可能是动脉硬化闭塞症发生发展的一个关键因素。

Expressions and Effects of LOX-1 in Type 2 Diabetes Mellitus Accompanying Lower Extremity Atherosclerotic Obliterans

Objective:To observe the expressions of LOX-1 mRNA and protein levels and explore its underlying pathogenesis in the patients with type 2 diabetes mellitus accompanying lower extremity atherosclerotic obliterans.Method:The arterial specimens from 9 cases of arteriosclerosis obliterans with diabetes mellitus(diabetes mellitus group)and 7 cases of normal kidney donors(control group)were detected with HE staining.The expressions of LOX-1 mRNA and protein levels were determined by reverse transcription-polymerase chain reaction(RT-PCR)and Western-blot to evaluate their changes of aortas homogenate.Results:Compared with the control group,LOX-1 mRNA and protein expressions were prominently increased in the group of diabetes mellitus(P<0.05).Conclusion:Lower extremity with type 2 diabetes mellitus has higher risks of arteriosclerosis obliterans than normal group by up-regulating the expressions of LOX-1.LOX-1 may be one of the key factors for the development and progression of atherosclerosis obliterans initiated.