肝肺综合征大鼠血浆和肺组织ET-1的动态变化及其基因表达

目的 :探讨ET 1在肝肺综合征 (HPS)发生机制中的作用。方法 :采用Wistar大鼠行胆总管结扎术 (CBDL)制备HPS动物模型 ,抽取动脉血进行血气分析 ,使用放射免疫方法检测血浆和肺组织中ET 1水平 ,采用RT PCR方法检测肺组织中ET 1前体原 (ppET 1)mRNA的表达。结果 :CBDL组大鼠血浆和肺组织ET 1含量随时间延长而逐渐升高 ,CBDL各组血浆ET 1水平与假手术组 (Sham组 )比较差异有显著意义 (P <0 .0 5～ 0 .0 1) ;CBDL5周组肺组织ET 1含量及肺组织ET 1前体原 ( ppET 1)mRNA与Sham组比较差异有显著意义 (P <0 .0 5～ 0 .0 1) ;相关分析表明 ,血浆和肺组织中ET 1与肺泡动脉氧分压差A aDO2 显著相关 (P <0 .0 1)。结论 :在HPS形成过程中 ,血浆和肺组织中ET -1水平升高参与了HPS的发生

Gene Expression and Dynamic Changes of ET-1 in Plasma and Lung Tissue on Hepatopulmonary Syndrome in Rats

Objective: To explore the role of ET 1 in the mechanism of hepatopulmonary syndrome(HPS).Method: Wistar rats were used and common bile duct ligation(CBDL) were performed. At 3 weeks、4 weeks and 5 weeks after the operation, when the rats were killed while obtained blood from abdominal aorta for evaluation of arterial blood gases.The blood and lung tissue sample were collected to determine the plasma and lung tissue homogenate ET 1 levels by radioimmunoassay; Using specific primers, RT PCR were performed to assess the pulmonary ppET 1mRNA levels.Results: The plasma and pulmonary ET 1 levels are dramatically increased from 3 week to 5 week after CBDL. There are significant difference of plasma ET 1 between CBDL groups and control( P < 0.05 ～0.01), The lung tissue ET 1 and ppET 1 mRNA levels of CBDL5 week group are higher than control group( P <0.05～0.01). Plasma and lung ET 1 correlated with A aDO 2 positively( P <0.01).Conclusion: During the forming of HPS,the level of ET 1 of plasma and lung tissue increased and related with the abnormal of gas exchange,ET 1 may contribute to the mechanism of HPS.