小鼠受精卵显微操作方法的改进

目的：改进小鼠受精卵显微操作方法,提高小鼠受精卵的存活率、假孕母鼠生产率及得仔率。方法：以一定长度大小及浓度的DNA片段为材料,分别进行常规及改进的显微操作后,统计分析受精卵存活率、假孕母鼠生产率及得仔率。结果：经改进后缩短操作时间的显微操作法与常规显微操作法相比,小鼠受精卵的存活率由60．3％提高至79．5％、假孕母鼠生产率由35．7％提高至56．8％、得仔率由10．1％提高至14．1％,均有显著差别（P＜0．01）。结论：改进的显微操作法可推广应用于常规的转基因小鼠制备。

Improvement on micro-manipulation of fertilized eggs of mice

Objective: To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice. Methods: DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared. Results: With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively. Conclusion: The improved methods of micro-manipulation can be used in transgenic mouse production.