| HPLC法测定大鼠血浆中昂丹司琼含量 |
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| 引用本文： | 李文锋,麻伟青,杨云丽,董发团,普英杰.HPLC法测定大鼠血浆中昂丹司琼含量[J].昆明医学院学报,2011,32(5):24-26. |
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| 作者姓名： | 李文锋 麻伟青 杨云丽 董发团 普英杰 |
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| 作者单位： | 1. 成都军区昆明总医院麻醉科,云南,昆明,650032
2. 解放军第533医院药械科,云南,昆明,650224 |
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| 摘 要： | 目的建立高灵敏度HPLC法测定大鼠血浆中昂丹司琼含量.方法大鼠血浆样本用乙酸乙酯液-液萃取后,采用Waters Atlantis C18（150 mm×4.6 mm,5μm）色谱柱,以奈韦拉平为内标,流动相为乙腈：0.002μL/mL磷酸二氢钾缓冲液（用磷酸调pH到3.3）=30：70,流速1.0 mL/min,柱温35℃,检测波长310nm.结果血浆中昂丹司琼2～1 000 ng/mL范围内线性关系良好,批内、批间精密度分别〈5.1%和7.7%,昂丹司琼提取回收率为90.4%～94.1%.结论 HPLC方法快速、灵敏、准确,适用于昂丹司琼血浆浓渡的测定及其药理实验研究.
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| 关 键 词： | 昂丹司琼 HPLC 含量测定 |
Determination of Ondansetron in Rats Plasma by HPLC |
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| LI Wen-feng,MA Wei-qing,YANG Yun-li,Dong Fa-tuan,PU Ying-jie.Determination of Ondansetron in Rats Plasma by HPLC[J].Journal of Kunming Medical College,2011,32(5):24-26. |
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| Authors: | LI Wen-feng MA Wei-qing YANG Yun-li Dong Fa-tuan PU Ying-jie |
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| Institution: | LI Wen-feng1),MA Wei-qing1),YANG Yun-li1),Dong Fa-tuan1),PU Ying-jie2)(1)Dept.of Anesthesiology,Kunming General Hospital of Chengdu Military Command,Kunming Yunnan 650032,2)Drug and Equipment Section,The 533 Hospital of PLA,Kunming Yunnan 650224,China) |
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| Abstract: | Objective To establish high-performance liquid chromatographic（HPLC）method for the quantification of ondansetron in rats plasma.Methods Sample preparation was accomplished through liquid-liquid extraction with ethyl acetate and chromatographic separation was performed on a reversed phase Waters Atlantis C18 column at 35 ℃.Mobile phase consisted of a mixture of acetonitrile-0.02 μL/mL KH2PO4 buffer（pH 3.3）（30：70,v/v）at a flow rate of 1.0 mL/min.Wavelength was set at 310 nm.Helicid was selected as the internal standard（IS）.Results The linear range of ondansetron in plasma was 2～1000 ng/mL.The intra-day and inter-day precisions were lower than 5.1 and 7.7%,respectively.The recovery rate of ondansetron was 90.4～94.1%.Conclusion HPLC is rapid,sensitive and accurate,which can be used for determination of andansetron in rat plasma and its pharmacology study. |
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| Keywords: | HPLC |
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