H2O2诱导骨骼肌卫星细胞凋亡及法舒地尔的保护作用

背景：骨骼肌卫星细胞是成体骨骼肌中位于肌细胞膜和基膜之间具有增殖分化潜能的肌源性干细胞，研究表明骨骼肌卫星细胞的有效性及安全性，但是移植后的干细胞成活率极低，极大的限制了骨骼肌卫星细胞的应用。目的：观察过氧化氢(H₂O₂)对大鼠骨骼肌卫星细胞凋亡的影响以及法舒地尔的保护作用。方法：取体外培养的骨骼肌卫星细胞，随机分为正常对照组，H₂O₂组，H₂O₂⁺法舒地尔组(法舒地尔组)，采用流式细胞仪检测细胞凋亡率，ELISA法检测白细胞介素4、肿瘤坏死因子a的浓度，Western blot检测Bax蛋白的表达。 结果与结论：同H₂O₂组相比，法舒地尔组凋亡发生率明显下降(P < 0.05)，Bax蛋白水平表达明显降低(P < 0.05)，白细胞介素4、肿瘤坏死因子a的分泌显著减少(P < 0.05)。结果提示，法舒地尔抑制Rho激酶信号途径发挥抗凋亡保护作用，其机制可能与减少Bax蛋白的表达有关。 

Fasudil inhibits apoptosis of skeletal muscle satellite cells induced by H2O2

BACKGROUND:Skeletal muscle satellite cells are muscle-derived stem cells with proliferation and differentiation potential distributing between the muscle cell membrane and the base film. Studies have shown that skeletal muscle satellite cells are of efficacy and safety, but the survival rate of the transplanted stem cells is very low, which greatly limits the application of skeletal muscle satellite cells.OBJECTIVE:To observe the effects of Fasudil on apoptosis of skeletal muscle satellite cells induced by H₂O₂. METHODS:Skeletal muscle satellite cells cultured in vitro were randomly divided into three groups including H₂O₂ group, H2O2+Fasudil group (Fasudil group) and control group. Apoptosis rates were observed by flow cytometry. The concentrations of interleukin-4 and tumor necrosis factor-a in each group were detected by ELISA. Western blot was employed to measure the protein level of Bax in each group. RESULTS AND CONCLUSION:Compared with the H₂O₂ group, a significant decrease was found in the apoptosis rate of cells, protein level of Bax, and concentrations of interleukin-4 and tumor necrosis factor-a in the Fasudil group (all P < 0.05). These findings indicate that Fasudil can play anti-apoptosis protection by inhibiting Rho-kinase signaling pathway, which may be related to the reduced expression of Bax.