昆明市VCT人群中的HIV-1亚型和耐药基因型调查

摘要:目的　了解昆明市艾滋病自愿咨询检测（VCT）人群中的艾滋病病毒（HIV）的HIV-1亚型和耐药基因型分布情况。方法　于2013年11-12月在云南省疾病预防控制中心VCT门诊对就诊人群进行调查，并采样进行HIV抗体、CD4细胞、新发感染检测和HIV亚型和耐药基因型检测。结果　610例VCT样本中有96例为HIV抗体阳性（15.7%，96/610），其中95例（99.0%，95/96）获得亚型分析结果，分别有CRF08_BC（40.0%，38/95）、CRF07_BC（27.4%，26/95）、CRF01_AE（27.4%，26/95）、C（4.2%，4/95）和B（1.1%，1/95）5种亚型。89例样本（92.7%，89/96）获得耐药基因型分析结果，有21例（23.6%，21/89）发生基因突变，有1例出现耐药传播突变位点（1.12％，1/89）。结论　VCT人群中的艾滋病病毒亚型以重组流行型为主，包括CRF08_BC、CRF07_BC和CRF01_AE。耐药毒株在该人群中为低流行。

The survey of HIV-1 subtypes and drug resistance mutations among people who seeking VCT service in Kunming

Abstract: Objective The aim of this study was to determine HIV-1 subtypes and drug resistance mutations among HIV infected subjects found in the HIV voluntary counseling and testing (VCT) clinic in Kunming. Methods Subjects from the VCT clinic in Yunnan center for disease control and prevention were investigated and blood samples were taken to test HIV antibody, CD4 counts, BED incidence test, HIV-1 subtypes and drug resistance genes in November and December 2013. Results Ninety-six were confirmed as positive of HIV antibody from 610 samples (15.7%, 96/610). HIV-1 subtypes were determined from 95 subjects, which were CRF08_BC (40.0%, 38/95), CRF07_BC (27.4%, 26/95), CRF01_AE (27.4%, 26/95), C (4.2%, 4/95) and B (1.1%, 1/95). Drug resistance genes were studied from 89 subjects and 21 were found with mutations (23.6%, 21/89) while only one was related to drug resistance mutations (1.12%, 1/89). Conclusion HIV-1 subtypes in VCT population were mainly circulating recombinant forms (CRFs) which were CRF08_BC, CRF07_BC and CRF01_AE. The prevalence of HIV drug resistance strains were slowly epidemic in VCT population.