Epstein-Barr病毒基因表达在儿童SLE中作用的研究

摘要:目的　通过研究EB病毒（EBV）基因在儿童系统性红斑狼疮（SLE）中的表达，探讨EBV在儿童SLE发病中的作用。方法　（1）分离20例SLE患儿和12例健康对照外周血单核细胞（PBMCs）。（2）用ELISA检测EBV-IgG/IgM阳性的SLE患儿，取其分泌EBV病毒的细胞上清液与提取的PBMCs共同培养12 d，提取培养后的PBMCs的RNA，用逆转录实时定量PCR（RT-PCR）的方法检测EBV基因的表达。结果　SLE患儿组伏期基因LMP1、LMP2、EBNA-1和裂解期基因BCRF1、BLLF1的表达均显著高于健康对照组，差异有统计学意义（分别为1.011±0.145比0.383±0.180，t＝10.680，P＜0.05；1.001±0.096比0.208±0.086，t＝25.109，P＜0.05；1.012±0.168比0.188±0.157，t＝14.591，P＜0.05；1.015±0.171比0.344±0.169，t＝10.680，P＜0.05；1.038±0.271比0.117±0.085，t＝10.680，P＜0.05）。结论　儿童SLE患者中存在EBV基因的异常表达，EBV基因可能参与了SLE的发病过程。

Aberrant expression of Epstein-Barr Virus Genes in children systemic lupus erythematosus

Abstract: Objective This work was to discuss the role of Epstein-Barr Virus (EBV) in the pathogenesis of children Systemic Lupus Erythematosus (SLE) through EBV Gene expression examination. Methods Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 20 patients with SLE and 12 healthy control subjects respectively. From children with SLE who were EBV-IgG/IgM positive by ELISA, samples were withdrawn to extract the supernatant of cells secreting EBV virus, which was co-cultured with PBMCs for 12 days. RT-PCR was applied to detect the expression of EBV genes. Results After the co-culture of PBMCs with cell supernatant containing EBV virus, the expression of latent EBV genes and lytic genes were both increased in patients and controls. The latent EBV genes including LMP1, LMP2, EBNA-1 and the lytic genes including BCRF1、BLLF1 were all increased significantly in patients compared to that in the controls (1.011±0.145 vs.0.383±0.180, t=10.680, P<0.05; 1.001±0.096 vs. 0.208±0.086, t=25.109, P<0.05; 1.012±0.168 vs. 0.188±0.157, t=14.591, P<0.05; 1.015±0.171 vs. 0.344±0.169, t=10.680, P<0.05; 1.038±0.271 vs. 0.117±0.085, t=10.680, P<0.05, respectively). Conclusion The aberrant expression of EBV genes in children SLE patients suggest that EBV genes may contribute to the pathogenesis of SLE.