2011-2014年常州地区HIV-1抗体阳性者免疫印迹带型分析

摘要:目的　分析2011-2014年常州地区HIV-1抗体阳性者的免疫印迹（Western Blot，WB）带型。方法　应用WB试验对2011-2014年常州地区HIV-1抗体阳性待确证标本进行确证检测，并对其WB带型进行统计学分析。结果　1144份HIV-1抗体待确证标本中，36份确证为阴性，71份为不确定，1037份为阳性，阳性率为90.7%。在HIV-1抗体阳性标本WB带型中，gp160阳性率最高，为96.5%；p24和gp120次之，阳性率均在90.0%以上；gp41、p66、p51和p31阳性率较低，均低于90.0%；p17、p55和p39阳性率分别为66.4%、45.7%和41.9%；WB带型阳性率男女性之间差异均无统计学意义（P＞0.05）；20～39岁组的p39阳性率高于其他年龄组（χ2＝10.861，P＜0.05），＜20岁组的p17阳性率高于其他年龄组（χ2＝7.906，P＜0.05）。结论　p39和p17带型阳性率在4个年龄组之间的差异均有统计学意义；gp160、gp120、gp41和p24是HIV-1抗体确证阳性的重要条带，且本地区检测的HIV-1抗体阳性者多在AIDS期前。

Analysis on the western blot band patterns of HIV-1 antibody positive samples in Changzhou from 2011 to 2014

Abstract: Objective This work was to analyze the western blot (WB) band patterns of HIV-1 antibody positive samples in Changzhou from 2011 to 2014. Methods HIV-1 antibody was detected by WB and the WB band patterns were analyzed statistically. Results Among 1144 HIV-1 specimens, 1037 were confirmed positive, accounting for 90.7%; 71 cases were uncertain and 36 cases were negative. Among 1037 positive samples, gp160 was expressed in 96.5% of the samples, p24 and gp120 were expressed over 90.0% of the samples, gp41, p66, p51 and p31 were expressed under 90.0% of the samples, p17, p55 and p39 were expressed in 66.4%, 45.7% and 41.9% of the samples, respectively. The positive rates of WB band patterns were not different between males and females. The positive rate of p39 in 20~39 years old group was higher than that in other groups (χ2=10.861, P<0.05), and the positive rate of p17 in the group under 20 years old was higher than that in other groups (χ2=7.906, P<0.05). Conclusion The expression of p39 and p17 WB band patterns was statistically different among four age groups. It was important band patterns of gp160, gp120, gp41 and p24 for confirming HIV-1 antibody positive. Most of HIV-1 antibody positive people in Changzhou were detected before AIDS period.