肺癌耐药相关基因在肺癌组织和肺癌细胞株中的表达

背景与目的：我们已经通过抑制消减杂交技术发现了一条新的长494bp的肺癌耐药相关基因片段,并克隆了它的全长cDNA序列。本研究旨在研究该肺癌耐药相关基因（lung cancer drug resistance-related gene,LCDRG）在肺癌组织、癌旁组织及5种肺癌细胞株中的表达。方法：应用半定量RT－PCR检测38例肺癌组织、12例癌旁组织和5种肺癌细胞株中该基因的表达。结果：肺癌组织中LCDRG mRNA的表达明显高于癌旁组织（P＜0．001）,但在肺腺癌和鳞癌之间差异无统计学意义。该基因在肺腺癌细胞株SPC－A－1和A549、大细胞肺癌细胞株H460、小细胞肺癌细胞株H446和SH77中的表达依次递减。结论：LCDRG是一条肺癌相关基因,该基因可能与肺癌的发生、发展密切相关。

Expression of a new lung cancer drug resistance-related gene in lung cancer tissues and lung cancer cell strains

BACKGROUND & OBJECTIVE: A new drug resistance related gene fragment which was 494 bp long was found using suppression subtractive hybridization (SSH) and its full length cDNA fragment was cloned by the authors. This study was designed to determine the expression of this lung cancer drug resistance related gene (LCDRG) in lung cancer tissues, juxtacancerous tissues, and five lung cancer cell strains. METHODS: The expression of LCDRG was determined by semi quantitative reverse transcription polymerase chain reaction (RT PCR) method in 38 lung cancer tissues,12 juxtacancerous tissues, and 5 lung cancer cell strains. RESULTS: The expression of LCDRG in cancer tissues was significantly higher than that in juxtacancerous tissue (P< 0.001). There was no significance of the expression among adenocarcinoma, brochioloalveolar carcinoma, and squamous carcinoma. In lung cancer cell strains, the expression levels of LCDRG in adenocarcinoma cell strains SPC A 1 and A549, big cell lung cancer cell strain H460, small cell lung cancer cell strains H446 and SH77 were decreased gradually. CONCLUSION:LCDRG is closely related to lung cancer and may be involved in the pathogenesis of lung cancer.