Demonstration of gene-amplification by PCR in archival paraffin-embedded breast cancer tissue |
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| Authors: | Ulf Lönn Sigrid Lönn Bo Nilsson Claes Silfverswärd Björn Stenkvist |
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| Affiliation: | (1) Radiumhemmet, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden;(2) Dept of Cancer Epidemiology, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden;(3) Dept of Tumor Pathology, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden;(4) Div of Cytology, Dept of Tumor Pathology, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden |
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| Abstract: | ![]()
Summary The polymerase chain reaction (PCR) is a powerful tool to examine genetic alterations in tumor samples. We describe a simple, rapid, nonisotopic PCR method to semi-quantitatively determine the number of gene copies in human formalin-fixed paraffin-embedded tissue. The procedure is exemplified by analysis of 15 years old breast cancer samples to determine the presence of amplification of c-erb-B2. The samples were obtained from routine specimens kept in pathological archives. Patients with amplified samples showed a poor prognosis, both for recurrences and death in breast cancer. |
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| Keywords: | breast cancer gene amplification PCR prognosis |
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