高加索人和日本人胰腺癌基因组杂合性缺失比较

目的研究胰腺癌细胞基因组范围内的杂合性缺失(LOH),比较高加索人和日本人的异同。方法应用高密度单核苷酸多态性芯片和分析软件,研究12种高加索人和8种日本人胰腺癌细胞株基因组范围内的LOH。结果每一种胰腺癌细胞基因组中都有不同程度的LOH,其中T3M-4细胞频率最高(54.5%);高加索人和日本人胰腺癌细胞株不同染色体臂出现LOH频率不同,高加索人中最常见的LOH是9p(12/12,100.0%),而日本人出现频率最高的是13q(7/8,87.5%)。高频率的(50%以上)共同的LOH出现在染色体臂3p,8p,9p,13q,17p,18q和22q。结论胰腺癌全基因组范围内出现多处LOH。不同种族LOH出现可能频率不同,高频率的杂合性缺失区域可能含有新抑癌基因,为今后胰腺癌的研究提供了新的线索和方向。

Comparison of Loss of Heterozygosity Between Caucasian and Japanese Pancreatic Cancer Cell Lines

Objective To compare the genomic-wide loss of heterozygosity between Caucasian and Japanese pancreatic cancer cell lines.Methods The genome-wide loss of heterozygosity in 12 pancreatic cancer cell lines originated from Caucasian and 8 from Japanese were analyzed by using high-density single nucleotide polymorphism array.Results Each pancreatic cancer cell line had at least one loss of heterozygosity,and T3M-4 cell had the highest frequency(54.5%).The different chromosome arms had different frequency of LOH on cells from Caucasian and Japanese.The most common abnormalities mapped to 9p(12/12,100.0%)in Caucasian,and 13q(7/8,87.5%)in Japanese.The common LOH with high frequency(larger than 50%)mapped to 3p,8p,9p,13q,17p,18q and 22q.Conclusion The loss of heterozygosity is one of the common events in pancreatic cancer cell.Different races might have different frequency.The high frequency regions might have novel tumor suppressor gene,which might provide new clues for the study on pancreatic cancer.