表皮生长因子减少腹部辐射肠外营养大鼠肠道细菌移位

观察表皮生长因子对接受长期全肠外营养的腹部辐射大鼠肠道细菌移位的影响,探讨其机制。方法应用ＴＳＢ培养,图像分析,高效液相色谱等方法检测应用ＧＥＦ后大鼠死亡率,肠粘膜形态结构,肠道细菌移位,血浆及组织谷氨酰胺水平及肠道Ｇｌｎ摄取率。结果应用ＥＧＦ后,大鼠钫率显降低,肠粘膜结构屏障改善,细胞移位减少,血液及组织Ｇｌｎ水平提高。结论ＥＧＦ在长期菌ＴＰＮ显改善辐射性肠粘膜屏障损伤大鼠营养的同时,减少Ｔ

Epidermal growth factor reduces gut bacterial translocation in parenterally fed rats following abdominal radiation

AIM To investigate the effects of EGF on bacterial translocation from gut in parenterally fed rats following abdominal radiation and to study its mechanism. METHODS The TSB culture, image processing and analysis system, and HPLC were employed for the morphological evaluation of gut mucosa, bacterial translocation from gut, glutamine content in blood and tissue, and gut glutamine extraction. RESULTS When EGF was administrated along with TPN in the rat following abdominal radiation, EGF could increase the glutamine content in blood and tissue and gut glutamine extraction significantly, improve gut mucosa barrier function, decrease the bacterial translocation from gut and decrease mortality. CONCLUSION While TPN offered the abdominal radiation rats an satisfactory nutrition, the EGF can increase the gut glutamine extraction and utilization and improve gut mucosa barrier function. Thus the mortality of TPN fed rats following abdominal radiation can be reduced.