当归红芪超滤膜提取物对急性心肌缺血大鼠AST、LDH和LDH1的影响

目的:探讨当归红芪超滤物对急性心肌梗死大鼠天冬氨酸氨基转移酶(aspartate aminotrans-ferase,AST),乳酸脱氢酶(actate dehydrogenase,LDH)和乳酸脱氢酶同工酶(lactate dehydrogenase,LDH1)活性的影响,阐明当归红芪超滤物对缺血心肌的保护作用。方法:结扎左前降支建立大鼠心梗模型。将80只雄性Wistar大鼠随机分成5组,每组16只,分别为造模组(myocardial infarction,MI)、假手术组(sham-operation,SH)、低剂量组(low-dose drug,LD)、中剂量组(middle dose group,MD)、高剂量组(high-dose drug,HD),分别检测给药24h和72h后外周血AST、LDH和LDH1的活性。结果:与SH组比较,MI组大鼠可见血清AST、LDH及LDH1活性明显增高(P0.05);与MI组比较,药物各剂量组AST、LDH及LDH1活性明显降低(P0.05),且峰值前移。结论:当归红芪超滤物可通过抗氧化作用降低AST、LDH及LDH1活性从而抗心肌缺血损伤,对大鼠心肌有明显的保护作用,且与用药时间及剂量密切相关。

Effect of Ultra-filtration Extract Mixture from Angelica Sinensis and Radix Hedysari on AST,LDH and LDH1-Acute Myocardial Ischemia Rats

Objective:To study the effect of ultra-filtration extract mixture from Angelica Sinensis and Radix Hedysari on activity of aminotransferase(AST),lactate dehydrogenase(LDH) and lactate dehydrogenase isoenzyme(LDH1) in serum of acute myocardial ischemia rats.Methods:Eighty healthy male wistar rats were randomly divided into five groups as Sham group,myocardial ischemia group,myocardial ischemia treated with low-dose,middle-dose and high-dose group;Rat myocardial ischemia model was established by ligation of the left anterior descending of coronary artery;the Angelica Sinensis and Radix Hedysari extract were obtained by ultrafiltration membrane technology;the activity of AST,LDH and LDH1 in serum was detected by using spectrophotometry after treatment for 24h and 72h independently.Results:Compared with the sham group,the myocardial ischemia group showed that the activity of AST,LDH and LDH1 in serum was significantly increased(P<0.05);the activity of AST,LDH and LDH1 in serum for each myocardial ischemia group treated with different doses of ultra-filtration extract mixture from Angelica Sinensis and Radix Hedysari was significantly decreased(P<0.05) compared with the myocardial ischemia group.Conclusion:The result shows that ultra-filtration extract mixture from Angelica Sinensis and Radix Hedysari can reduce the activity of AST,LDH and LDH1 in serum and have significantly protective effect on myocardial ischemia while showing a dose-effect and time-effect relationship.