栽培滇龙胆药材不同极性部位紫外吸收光谱特征与判别分析

目的:建立滇龙胆药材不同极性部位的紫外指纹图谱,分析不同栽培模式下药材光谱特征的差异。方法:采用紫外分光光度法检测样品的紫外吸收光谱数据,经过4点平滑和一阶求导对光谱数据进行预处理,建立紫外指纹图谱。运用共有峰率和变异峰率双指标序列分析法对不同栽培模式下滇龙胆药材的指纹特征及相似性进行评价,通过判别分析评价紫外指纹图谱区分药材栽培模式的能力。结果:不同栽培模式滇龙胆紫外指纹图谱在221~247 nm图谱重叠率低,呈现明显指纹特征;相同栽培模式下,滇龙胆醇提液与水提液紫外指纹图谱有差异,呈现药材不同极性部位的指纹特征。荒坡栽培和龙胆与木瓜间作2种模式下,药材化学成分的光谱特征较接近;荒坡栽培和龙胆与旱冬瓜间作模式下,药材化学成分变化较大,二者光谱特征具明显差别。来自不同栽培模式的滇龙胆样品90%能被正确识别。结论:滇龙胆药材不同极性部位紫外指纹图谱可协助区分药材栽培模式,为不同栽培模式下药材质量的等同性研究提供相关数据信息。

Ultraviolet Absorption Spectrum Characteristic and Discriminant Analysis of Different Polar Parts from Root and Stem of Cultivated Gentiana rigescens

Objective: To build UV fingerprint of different polar parts from root and stem of Gentiana rigescens and analyze difference of spectral characteristics among eight cultivation patterns. Method: UV data of samples was finished by UV spectrophotometry,data was processed by four print smooth and first orders.Similarly evaluation of UV fingerprint of G. rigescens medicinal materials from different cultivation patterns was finished by common peak ratio and variation peak ratio dual index sequence analysis.Discriminant analysis was used to evaluate discriminate ability of UV fingerprint data for cultivation pattern of G. rigescens. Result: Fingerprint of samples from eight cultivation patterns were lower similarly at 221-247 nm.Chemical polarity fingerprint of different samples,cultivated by same method,was different too.There were significant fingerprint characteristic.UV fingerprints were similar between G. rigescens which grown on slope of valley and which cultivated under Chaenomeles sinensis.However,chemical compounds were significant different between plant grown on slope of valley and which cultivated under Alnus nepalensis.Discriminant analysis showed that about 90% sample could be discriminated according to cultivation patterns. Conclusion: UV fingerprint of different polar parts from root and stem of G. rigescens can help to discriminate cultivation patterns of medicinal materials.