Identification of potential therapeutic target genes,key miRNAs and mechanisms in oral lichen planus by bioinformatics analysis |
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| Institution: | 1. Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, Jinan, Shandong 250021, China;2. Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People''s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China;3. Shandong Provincial ENT Hospital, Shandong Provincial ENT Hospital Affiliated to Shandong University, Jinan, Shandong 250012, China;4. Department of gastroenterology, Provincial Hospital Affiliated to Shandong University, Jinan, China;5. Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, Shandong University, Jinan 250061, China;6. School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia;1. Master''s Program in Orofacial Pain and Oral Medicine, Herman Ostrow School of Dentistry of University of Southern California, 925 W 34th St. Los Angeles, CA 90089, USA;2. Associate Professor, Division of Geriatrics, Special Patients and Behavioral Dentistry, Herman Ostrow School of Dentistry of University of Southern California, 925 W 34th St. Los Angeles, CA 90089, USA;3. Assistant Professor of Clinical Dentistry, Division of Periodontology, Diagnostic Sciences and Dental Hygiene, Herman Ostrow School of Dentistry of University of Southern California, 925 W 34th St. Los Angeles, CA 90089, USA |
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| Abstract: | The study aimed to identify the potential target genes and key miRNAs as well as to explore the underlying mechanisms in the pathogenesis of oral lichen planus (OLP) by bioinformatics analysis. The microarray data of GSE38617 were downloaded from Gene Expression Omnibus (GEO) database. A total of 7 OLP and 7 normal samples were used to identify the differentially expressed genes (DEGs) and miRNAs. The DEGs were then performed functional enrichment analyses. Furthermore, DEG-miRNA network and miRNA-function network were constructed by Cytoscape software. Total 1758 DEGs (598 up- and 1160 down-regulated genes) and 40 miRNAs (17 up- and 23 down-regulated miRNAs) were selected. The up-regulated genes were related to nuclear factor-Kappa B (NF-κB) signaling pathway, while down-regulated genes were mainly enriched in the function of ribosome. Tumor necrosis factor (TNF), caspase recruitment domain family, member 11 (CARD11) and mitochondrial ribosomal protein (MRP) genes were identified in these functions. In addition, miR-302 was a hub node in DEG-miRNA network and regulated cyclin D1 (CCND1). MiR-548a-2 was the key miRNA in miRNA-function network by regulating multiple functions including ribosomal function. The NF-κB signaling pathway and ribosome function may be the pathogenic mechanisms of OLP. The genes such as TNF, CARD11, MRP genes and CCND1 may be potential therapeutic target genes in OLP. MiR-548a-2 and miR-302 may play important roles in OLP development. |
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| Keywords: | Oral lichen planus Differentially expressed genes Differentially expressed miRNA Bioinformatics analysis |
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