Calcitonin induces collagen synthesis and osteoblastic differentiation in human periodontal ligament fibroblasts |
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| Affiliation: | 1. Blond McIndoe Laboratories, Room 3.107, Stopford Building, University of Manchester, Manchester M13 9PT, UK;2. Blond McIndoe Laboratories, Institute of Inflammation & Repair, Room 3.106, Stopford Building, University of Manchester, Manchester M13 9PT, UK;3. Centre for Tissue Injury and Repair, Institute of Inflammation and Repair, Room 1.536, Stopford Building, University of Manchester, Manchester M13 9PT, UK;4. Department of Oral & Maxillofacial Surgery, School of Dentistry, JR Moore Building, University of Manchester, Manchester M13 9PL, UK;1. Department of Otolaryngology, Changle People''s Hospital, Changle, 262400, China;2. Department of Stomatology, Changhai Hospital, The Second Military Medical University, Shanghai, 200433, China;3. Department of Otolaryngology-Head and Neck Surgery, Shanghai First Peoples Hospital, Shanghai Jiaotong University, Shanghai, 200080, China;1. School of Dentistry, Chung Shan Medical University, Taichung, Taiwan;2. Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan;3. School of Public Health, Chung Shan Medical University, Taichung, Taiwan;1. Department of Thoracic Surgery, Roswell Park Cancer Institute, Buffalo, New York;2. Department of Surgery, State University of New York at Buffalo, University at Buffalo, Buffalo, New York;3. Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York;4. Department of Pathology, Roswell Park Cancer Institute, Buffalo, New York;5. Department of Biostatistics and Bioinformatics, Roswell Park Cancer Institute, Buffalo, New York;1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. College of Marine Sciences, South China Agricultural University, Guangzhou 510642, PR China;4. Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China;5. State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, College of Marine Science, Hainan University, Haikou 570228, PR China |
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| Abstract: | BackgroundExtracellular matrix (ECM) secretion and osteogenic differentiation in periodontal ligament fibroblasts (PDLF) facilitate the neogenesis of alveolar bone, which is the cellular basis for alveolar bone repair. Calcitonin (CT) has been reported to play an important role in promoting ECM expression and inducing osteogenic differentiation in osteoblast, but its effects on PDLFs remain obscure.MethodsThe expression of CT, transforming growth factor-beta 1(TGF-β1) and bone morphogenetic protein (BMP) in gingival crevicular fluid (GCF) was measured by ELISA. The effects of CT on collagen synthesis and osteogenic differentiation in hPDLFs were investigated by using the primarily cultured hPDLFs infected with adenovirus carrying the CT gene. Gene expression was measured by quantitative PCR and western blot.ResultsThe expression of CT in gingival crevicular fluid (GCF) of patients with periodontitis was significantly higher than that of healthy subjects. In addition, CT expression correlated with the clinical indexes including probing pocket depth (PPD), clinical attachment level (CAL), and gingival index (GI). The in vitro study demonstrated that overexpression of CT by adenovirus infection increased the expression of TGF-β1, collagen type I and III, and osteoblastic markers including BMP-2/-4, alkaline phosphatase and osteocalcin in human PDLFs. Moreover, CT-enhanced collagen synthesis was abrogated in hPDLFs transfected with TGF-β1 siRNA, and CT-induced osteoblastic differentiation was blocked in hPDLFs by BMPs inhibitor noggin.ConclusionsThese results suggest that CT promotes collagen synthesis and osteogenic differentiation in hPDLFs via the TGF-β1 and BMPs signaling pathways, respectively. |
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| Keywords: | Calcitonin Periodontitis ECM expression Osteoblastic differentiation |
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