牙本质中可溶性磷蛋白对再矿化的抑制作用

目的 研究去除牙本质中可溶性磷蛋白后对进一步再矿化的影响，了解磷蛋白在牙本质矿化中的作用机制。方法 用EDTA脱矿法去除牙本质中的固有可溶性磷蛋白，获得脱矿的牙本质籽晶，进行再矿化实验，并与以正常牙本质和乙酸脱矿法（可保留可溶性磷蛋白）获得的牙本质再矿化过程对照。实验应用等组分晶体生长体系，记录再矿化过程添加液何种随时间的增加量，以计算再矿化速率。结果 经EDTA脱矿0.5h及2h的牙本质籽晶的再矿化速率比正常牙本质粉明显增快，在200min时观察到的速率增加可超过100%，而脱矿5h和10h以及所有乙酸脱矿牙本质籽晶的再矿化速率均较正常未脱矿籽晶慢。结论 牙本质固有的可溶性磷蛋白具有抑制脱矿牙本质再矿化的作用。

Inhibition of remineralization by EDTA-soluble phosphate protein in dentin

Objective To investigate the effect of removing EDTA soluble phosphate protein in dentin on the later remineralization for the purpose of better understanding of mechanism of dentin phosphate proteins on dentin mineralization Methods To remove soluble phosphate protein by EDTA dissolution, then the remineralization rate was monitored by a constant composition crystal growth technique The results were compared with those from the normal dentin and the dentin partially demineralized by acetic acid Results Faster remineralization rates were found with dentin demineralized by EDTA (0 5 and 2 h) compared with normal dentin powder, while a slower rate was found with dentin demineralized by acetic acid The increase of remineralization rate by removing phosphate protein from dentin was 100% more at 200 min after the start of the reaction Conclusion EDTA soluble phosphate protein in dentin has a great potential to inhibit remineralization