| 束缚-应激联合辣素灌胃建立大鼠腹泻型肠易激综合征模型及评价 |
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| 引用本文： | 张涛,方健松,黄晓燕,马媛萍,刘畅,潘锋. 束缚-应激联合辣素灌胃建立大鼠腹泻型肠易激综合征模型及评价[J]. 中国比较医学杂志, 2017, 27(3): 1-7 |
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| 作者姓名： | 张涛 方健松 黄晓燕 马媛萍 刘畅 潘锋 |
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| 作者单位： | 广西中医药大学附属瑞康医院消化科, 南宁 530011,广西中医药大学附属瑞康医院消化科, 南宁 530011,广西中医药大学第一附属医院消化科, 南宁 530011,广西中医药大学附属瑞康医院消化科, 南宁 530011,广西中医药大学附属瑞康医院消化科, 南宁 530011,浙江省中西医结合医院消化科, 杭州 310003 |
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| 基金项目： | 国家自然科学基金（NO：81460724）；广西自然科学基金（NO：2014GXNSFAA118212）。 |
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| 摘 要： | 目的基于观察大鼠症状学、组织病理学、内脏敏感性、肥大细胞活化、自噬、Beclin-1及Claudin-2等相关指标变化,评价束缚-应激联合辣素灌胃建立一种新的腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型研究。方法清洁级雄性SD大鼠40只,按体重分为正常组、模型I组、模型II组及模型III组,每组10只。模型组均采用束缚-夹尾应激联合辣素(I组0.125%、II组0.250%、III组0.500%)灌胃制备D-IBS模型,正常组予等剂量生理盐水灌胃处理,连续2周。分别应用Power lab仪检测大鼠腹壁收缩次数及弓背次数变化,肥大细胞染色液(醛品红-橙黄G法)检测肥大细胞活化情况,采用光镜、电镜检测大鼠结肠组织病理学及其自噬变化,应用免疫组化SABC法检测大鼠结肠黏膜Beclin-1及Claudin-2表达变化。结果模型III组大鼠全部死亡,模型II组大鼠大便次数增多,内脏敏感性阈值降低,与正常组及模型I组比较,差异有显著性(P0.05);各组大鼠结肠黏膜、黏膜上皮绒毛及腺体形态正常,未见黏膜下血管扩张及弥漫性炎症浸润改变;正常组除外,模型I组、II组大鼠结肠组织黏膜间质或黏膜下层可见类圆形紫红至深紫色染色点,提示肥大细胞表达增多;模型II组大鼠结肠肠上皮细胞自噬、Beclin-1及Claudin-2表达明显增多,与正常组及模型I组相比,差异有显著性(P0.05)。结论束缚-应激联合0.25%辣素灌胃建立的大鼠D-IBS模型以腹泻增多、内脏高敏感、肥大细胞表达增高、肠上皮细胞自噬增加、肠黏膜屏障破坏等表现为特点,与人IBS较为相似,该方法简单易行,值得推广应用。
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| 关 键 词： | 腹泻型肠易激综合征 束缚-应激 辣素 内脏敏感性 自噬 |
| 修稿时间： | 2016-07-15 |
Establishment and evaluation of a rat model of diarrhea-predominant irritable bowel syndrome by restraint-stress combined with capsaicin administration |
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| ZHANG Tao,FANG Jian-song,HUANG Xiao-yan,MA Yuan-ping,LIU Chang and PAN Feng. Establishment and evaluation of a rat model of diarrhea-predominant irritable bowel syndrome by restraint-stress combined with capsaicin administration[J]. Chinese Journal of Comparative Medicine, 2017, 27(3): 1-7 |
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| Authors: | ZHANG Tao FANG Jian-song HUANG Xiao-yan MA Yuan-ping LIU Chang PAN Feng |
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| Affiliation: | Department of Gastroenterology, Ruikang Hospital Affiliated of Guangxi University of TCM, Nanning 530011, China,Department of Gastroenterology, Ruikang Hospital Affiliated of Guangxi University of TCM, Nanning 530011, China,Department of Gastroenterology, The First Affiliated Hospital of Guangxi University of TCM, Nanning 530011,Department of Gastroenterology, Ruikang Hospital Affiliated of Guangxi University of TCM, Nanning 530011, China,Department of Gastroenterology, Ruikang Hospital Affiliated of Guangxi University of TCM, Nanning 530011, China and Department of Gastroenterology, The Zhejiang Hospital of Integrated Chinese medicine and Western Medicine, Hangzhou 310003 |
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| Abstract: | |
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| Keywords: | Irritable bowel syndrome Restraint stress Capsaicin Visceral hypersensitivity Autophagy |
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