细胞趋化因子受体-5和CD57在自身免疫性甲状腺疾病中的表达及意义

目的通过观察滤泡辅助T细胞（Tfh细胞）相关因子细胞趋化因子受体_5（CXCR5）及CD57在桥本甲状腺炎及Graves病患者甲状腺的表达，探讨个m细胞在自身免疫性甲状腺疾病发生中的作用。方法利用免疫组织化学sP法检测CXCR5、CD57在15例桥本甲状腺炎及18例Graves病患者甲状腺的表达，以10例正常甲状腺组织为对照组。结果CXCR5、CD57在桥本甲状腺炎及Graves病主要表达于浸润淋巴细胞的胞膜和胞质，CXCR5阳性率分别为93．3％（14／15）和88．9％（16／18），CD57表达的阳性率分别为93．3％（14／15）和83．3％（15／18），均显著高于正常对照（P均〈0．05），两种分子在桥本甲状腺炎和Graves病中的表达差异无统计学意义（P〉0．05）。结论CXCR5及CD57主要表达在甲状腺组织浸润的淋巴细胞的胞质和胞膜，且在两种自身免疫性甲状腺疾病中的表达呈现一致性，提示Tfh细胞参与了自身免疫性甲状腺疾病的发生。

The expression of CXCR5 and CD57 in autoimmune thyroid diseases

Objective To investigate the potential role of T follicular helper cells （Tfh） in the pathogenesis of autoimmune thyroid diseases by comparing the expression of C-X-C chemokine teceptor type 5 （CXCRS） and CD57 in Hashimoto＇s thyroiditis （HT） and Graves＇ disease （GD） thyroid tissues. Methods The expression of CXCR5 and CD57 proteins was determined by immunohistochemical analysis in 15 HT thyroid samples, 18 GD samples and 10 normal thyroid samples. Results Immunohistochemical staining showed that CXCR5 and CD57 were mainly positive in cytomembrane and cytoplasm of the infiltrated lymphocytes both in HT and GD tissues, with much higher levels than that of normal thyroid tissues （P 〈 0.05 ）. Both CXCR5 and CD57 were not significantly different between the HT and GD tissues. Conclusion CXCR5 and CD57 expressions were increased with a similar expression pattern in both of the two main autoimmune thyroid diseases（ AITD）, indicating that Tfh may participate in the development and progression of AITD.