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| 菟丝子黄酮对缺血再灌注大鼠心肌细胞凋亡的影响 | |
| 引用本文： | 翟宏颖,王桂敏. 菟丝子黄酮对缺血再灌注大鼠心肌细胞凋亡的影响[J]. 中国动脉硬化杂志, 2011, 19(3): 211-215 |
| 作者姓名： | 翟宏颖 王桂敏 |
| 作者单位： | 辽宁医学院附属第一医院中医科,辽宁省锦州市,121001 |
| 摘 要： | 目的观察菟丝子黄酮对缺血再灌注大鼠心肌细胞凋亡及相关基因表达的影响。方法结扎大鼠冠状动脉左前降支30 min,再灌注2 h建立心肌缺血再灌注模型。将50只SD大鼠随机分为假手术组、缺血再灌注组及菟丝子黄酮低、高剂量组和消心痛组。再灌注结束后检测血清肌酸激酶和乳酸脱氢酶,DNA末端标记法计算凋亡指数,免疫组织化学法和Western Blotting法检测心肌Bcl-2和Bax蛋白表达,计算Bcl-2/Bax值。结果与假手术组比较,缺血再灌注组心肌酶肌酸激酶、乳酸脱氢酶和凋亡指数显著增高(P<0.01),Bcl-2和Bax蛋白表达增强(P<0.01);与缺血再灌注组比较,菟丝子黄酮低、高剂量组及消心痛组能降低血清肌酸激酶、乳酸脱氢酶含量和凋亡指数(P<0.01),增加Bcl-2而减少Bax的表达(P<0.01)。结论菟丝子黄酮能够抑制缺血再灌注损伤大鼠的心肌细胞凋亡,与消心痛效果相近。 |
| 关 键 词： | 菟丝子黄酮 缺血再灌注损伤 细胞凋亡 Bcl-2蛋白 Bax蛋白 |
| 收稿时间： | 2010-10-27 |
Effect of Flavonoids from Cuscuta Chinensis on Myocardial Cells Apoptosis in Ischemic/Reperfused Rat Hearts |
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| ZHAI Hong-Ying,and WANG Gui-Min. Effect of Flavonoids from Cuscuta Chinensis on Myocardial Cells Apoptosis in Ischemic/Reperfused Rat Hearts[J]. Chinese Journal of Arteriosclerosis, 2011, 19(3): 211-215 | |
| Authors: | ZHAI Hong-Ying and WANG Gui-Min |
| Affiliation: | ZHAI Hong-Ying,and WANG Gui-Min(Traditional Chinese Medicine,the First Affiliated Hospital of Liaoning Medical College,Jinzhou,Liaoning 121001,China) |
| Abstract: | Aim To observe the effect of flavonoids from Cuscuta Chinensis on myocardial cell apoptosis and the related gene expression in ischemic/reperfused myocardial infarction. Methods The left anterior descended coronary artery was ligated for 30 min and reperfused for 2 h to make ischemia-reperfusion injury model.50 SD rats were randomly divided into the sham operation group,ischemia-reperfusion group,the low and high dose groups of flavonoids from Cuscuta Chinensis and isosorbide dinitrate group.Creatine kinase... |
| Keywords: | Flavonoids from Cuscuta Chinensis Ischemia-Reperfusion Injury Apoptosis Bcl-2 Protein Bax Protein |
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