A modified plaque test for the detection of cells forming antibody to alloantigens |
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| Authors: | G M Taylor J Bennett |
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| Affiliation: | Charles Salt Research Centre, The Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, SY10 7AG, England |
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| Abstract: | ![]()
Cells secreting antibody to H-2 alloantigens can be detected in vitro by the alloantibody plaque assay. This test has been modified to allow the earlier and more objective determination of plaques by staining the lysed target cells with trypan blue. Details are given of optimum conditions necessary for the assay. |
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