血清MMP-3、TIMP-1及HC gp-39在早期RA患者的表达与临床的相关性研究

目的：研究血清基质金属蛋白酶-3（matrix metalloproteinase 3,MMP-3）、金属蛋白酶组织抑制剂-1（tissue inhibitors of metalloproteinase 1,TIMP-1）及人类软骨糖蛋白-39（human cartilage glycoprotein 39,HC gp-39）在早期类风湿关节炎（rheumatoid arthritis,RA）患者的表达及其相关性因素,探讨其在早期RA的可能意义。方法：选取45例病程〈1年的早期RA患者和32例健康查体者,采用酶联免疫吸附试验（ELISA）分别测定血清MMP-3、TIMP-1及HC gp-39水平,同时测定关节肿胀数（SJC）、关节压痛数（TJC）、血沉（ESR）、C反应蛋白（CRP）、类风湿因子（RF）等相关实验室指标。结果：早期RA患者血清MMP-3、TIMP-1及HC gp-39水平明显高于正常对照组（P〈0.01）。不同X线分期各指标差异有统计学意义（P〈0.05）。不同关节功能分级时各指标差异无统计学意义（P〉0.05）。三指标与SJC（P〈0.01）、TJC（P〈0.01）、ESR（P〈0.01）、CRP（P〈0.01）呈正相关,与年龄、病程、晨僵时间、RF无明显相关（P〉0.05）。结论：MMP-3、TIMP-1及HC gp-39在早期RA患者血清中呈高水平表达,可作为反映疾病活动和早期RA患者出现骨侵蚀的有益预测指标。

Study on the Correlationship Between Serum Levels of MMP-3,TIMP-1,HC gp-39 and Clinical Activity in Patients with Early Rheumatoid Arthritis

Objective To investigate how the changes of serum levels of the matrix metalloproteinase 3（MMP-3）,tissue inhibitors of metalloproteinase 1（TIMP-1） and human cartilage glycoprotein 39（HC gp-39） might reflect the clinical activity and radiological damage in patients with early rheumatoid arthritis（RA）.Methods The serum levels of MMP-3,TIMP-1 and HC gp-39 were measured with ELISA in 45 patients with early RA and 32 controls.Results Significantly increased serum levels of MMP-3,TIMP-1 and HC gp-39 were found in early RA patients as compared with those in controls（P〈0.01）.The serun levels of MMP-3,TIMP-1 and HC gp-39 were positively correlated with the X-ray stage,SJC,TJC,ESR and CRP level（P〈0.05）,but not with age,the disease course,length of morning stiffness,stage of function and RF level（P〉0.05）.Conclusion Serum MMP-3,TIMP-1 and HC gp-39 levels increased significantly in early RA patients and might be used as indicators for disease activity and progress of bone erosion.