乳腺肿瘤基质金属蛋白酶2表达与肥大细胞的关系

目的]探讨乳腺肿瘤中基质金属蛋白酶2(matrix meta]]oproteinase2,MMP-2)表达及其与肥大细胞浸润的关系.方法]采用甲苯胺蓝染色显示55例良恶性乳腺肿瘤组织的肥大细胞,免疫组化EnVision法检测肿瘤细胞MMP-2以及类胰蛋白酶(tryptase)的表达.结果]乳腺癌间质成纤维细胞MMP-2的反应程度明显高于癌细胞.浸润性小叶癌和浸润性导管癌MMP-2的阳性率达86.7%,明显高于恶性程度较低的黏液腺癌和髓样癌(20.0%)以及乳腺的良性肿瘤(13.3%).局部有转移的乳腺癌组织中MMP-2的表达程度明显高于无转移组.乳腺肿瘤间质中肥大细胞的数量以及tryptase的表达量与MMP-2相似,但是与MMP-2的表达量并无明显的相关性.结论]MMP-2的表达是乳腺癌一个预后指标,而肥大细胞的增多可能是肿瘤纤维间质形成的相伴现象.

The Relationship Between Matrix Metalloproteinase-2 and Mast Cells in Breast Tumor

To investigate the relationship between matrix metalloproteinase 2(MMP-2) and mast cells in the breast tumor. The mast cells was detected by toluidne blue stain in paraffin-embeded tissue sections in 55 cases with breast tumor.MMP-2 and tryptase were detected by immunohistochemistry in the same tissue sections.The staining of MMP-2 was stronger in interstitial fibroblasts than that in tumor cells.MMP-2 was positive in 86.7% cases with invasive duct and lobular carcinoma, which was much higher than that in mucinous carcinoma(20.0%), medullary carcinoma20.0%,and other benign disease(13.3%). The expression of MMP-2 in cases with metastasis was stronger than that in cases without metastasis.The count of mast cells and the expression of tryptase in interstitial were found same as the expression of MMP-2 in interstitial,but no correlation between them.Conclusion]MMP-2 expression may be a useful prognostic factor in breast cancer, while an increase in mast cell number may be associated with fibro-interstitial formation.