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| 莪术油对人肺腺癌A549细胞中组织蛋白酶D,K表达的影响 | |
| 引用本文： | 杨长福,黄春芳,孙晓芳,牛建昭,王继峰. 莪术油对人肺腺癌A549细胞中组织蛋白酶D,K表达的影响[J]. 中国中药杂志, 2012, 37(6): 842-846 |
| 作者姓名： | 杨长福 黄春芳 孙晓芳 牛建昭 王继峰 |
| 作者单位： | 1. 贵阳中医学院,贵州贵阳,550002 2. 北京中医药大学,北京,100029 3. 河北大学,河北保定,071002 |
| 基金项目： | 科技部国际合作项目(2008DFA31970); 教育部高等学校学科创新引智计划项目(B07007);北京中医药大学自主选题项目(JYBZZ-JS023) |
| 摘 要： | 目的:探讨莪术油对A549细胞中胶原及组织蛋白酶D,K表达的影响。方法:用不同浓度的莪术油(0,40,80,120 mg.L-1)作用A549细胞,苦味酸天狼猩红染色法观察莪术油对A549细胞层胶原分泌的动态变化;酶法测定细胞中可溶性羟脯氨酸的分泌;Western blot检测Cat D,Cat K的表达情况。结果:莪术油刺激细胞后A549细胞分泌的胶原含量随剂量和作用的时间增加而减少。Cat D,Cat K蛋白的表达随作用时间持续而增强。结论:莪术油减少肺腺癌A549细胞胶原沉积,其机制可能与上调Cat D,Cat K蛋白表达有关。 |
| 关 键 词： | 莪术油 A549细胞 胶原 组织蛋白酶K 组织蛋白酶D |
| 收稿时间： | 2011-04-20 |
Effect of Zedoary oil for cat D and cat K expression in A549 cell line |
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| YANG Changfu,HUANG Chunfang,SUN Xiaofang,NIU Jianzhao and WANG Jifeng. Effect of Zedoary oil for cat D and cat K expression in A549 cell line[J]. China Journal of Chinese Materia Medica, 2012, 37(6): 842-846 | |
| Authors: | YANG Changfu HUANG Chunfang SUN Xiaofang NIU Jianzhao WANG Jifeng |
| Affiliation: | Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;Beijing University of Traditional Chinese Medicine, Beijing 100029, China;Hebei University, Baoding 071002, China;Beijing University of Traditional Chinese Medicine, Beijing 100029, China;Beijing University of Traditional Chinese Medicine, Beijing 100029, China |
| Abstract: | Objective: To explore the Zedoary oil on A549 cell line of collagen deposition cat D and cat K expression. Method: The A549 cell line were treat by Zedoary oil on four different concentrations (0, 40, 80, 120 mg·L-1) in different time. Dynamic changes of collagen in A549 cell using Picric-sirius red method. Cat D and Cat K expression of level were detected by using western blot. Result: The collagen content showed that Zedoary oil had an inhibitory effect on the deposition of A549 cells. The results of western blot showed that the expression of cat D and cat K were up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls. Conclusion: A549 cell of collagen deposition were reduced by Zedoary oil. The effects may due to the up-regulation of cat D and cat K. |
| Keywords: | Zedoary oil A549 cell collagen cat K cat D |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |