甘附冲剂抗大鼠心动过缓及其作用机制的研究

目的: 探讨甘附冲剂抗大鼠心动过缓的可能作用机制。 方法: 用普萘洛尔(Pro)制备大鼠心率减慢模型;测定大鼠心肌组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。放射免疫法测定心肌组织中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)及血浆中降钙素基因相关肽(CGRP)、内皮素(ET)的含量。 结果: 30,90,270 mg·kg^-1甘附冲剂可显著抑制Pro引起的大鼠心率减慢(P<0.05或P<0.01),和心肌组织中MDA含量的升高,提高大鼠血浆CGRP的含量,并可显著降低心肌组织中cGMP的含量,提高cAMP/cGMP比值(P<0.05或P<0.01);90,270 mg·kg^-1甘附冲剂可增强大鼠心肌组织中SOD和GSH-PX的活性(P<0.05);甘附冲剂各剂量对大鼠心肌组织中cAMP含量及大鼠血浆ET含量并没有明显影响。 结论: 甘附冲剂抗大鼠心动过缓的作用可能与抗自由基、升高CGRP及调节cAMP和cGMP的平衡有关。

The Study of Anti-bradycardic Effect and Mechanism of Ganfu Granule in Rats

Objective: To study the anti-bradycardic mechanism of Ganfu granule in rats. Methods: The bradycardic model in rats was induced by propranolol (Pro). the activity of glutathione peroxidase(GSH-PX),superoxide dismutase (SOD) and the content of Malonydialdehyde (MDA) in heart tissue were detected; the content of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) in heart tissue as well as the content of calcitonin gene-related peptide (CGRP),endothelin (ET) in plasma were detected by radioimmunoassay. Results: Ganfu granule (30,90,270 mg·kg^-1) significantly inhibited the Pro-induced decrease of heart rate in rats, reduced the content of MDA,cGMP in heart tissue, markedly increased the content of CGRP in plasma and the ratio of cAMP/cGMP in heart tissue. Gan granule(90, 270 mg·kg^-1) markedly increased the activity of SOD and GSH-PX in heart tissue; Compare with model group, the changes of the content of ET in plasma and cAMP in heart tissue were not obviouse. Conclusion: The anti-bradycardic mechanism of Ganfu granule may be related to anti-free radicals, increasing CGRP, regulating the balance between cAMP and cGMP.