大鼠脊髓缺血损伤后神经微丝及胶质纤维酸性蛋白的改变

目的神经微丝（ＮＦ）和胶质纤维酸性蛋白（ＧＦＡＰ）分别为神经细胞和胶质细胞特异的中间丝蛋白。着重探讨大鼠脊髓缺血损伤后ＮＦ和ＧＦＡＰ的改变及其可能的病理意义。方法利用ＮＦ和ＧＦＡＰ的单抗，借助于免疫组化、图像分析等手段，对大鼠腹主动脉再灌流缺血模型损伤脊髓神经细胞ＮＦ６８染色强度及胶质细胞数进行定性定量研究。结果伤后１天，ＮＦ的变化不明显；伤后３天，ＮＦ染色明显降低；７天达最低水平，并持续至２１天；２８天基本恢复至伤前。而胶质细胞数与正常相比，伤后１，３，７天无明显变化；至伤后１４天明显增加，并随时间而增加；至２８天达高峰。结论脊髓损伤后ＮＦ的降低与创伤性脑损伤中ＮＦ的变化相似，提示亦具有扩散性轴突损伤的存在。而胶质细胞的增生可能参与脊髓损伤的修复过程。

Changes of Neurofilament and Glial Fibrillary Acid Protein Following Ischemic Injury of Spinal Cord in Rats

Aim To study the changes of neurofilament (NF) and glial fibrillary acid protein (GFAP) and their pathological significance following ischemia of rat spinal cord. Methods Qualitative and quantitative changes of NF with immunohistochemical staining and the number of glial cells in ischemic spinal cord were studied by the monoclonal antibodies of NF68 and GFAP with immunohistochemistry and image analysis after occlusion of peritoneal aorta of rats for 30 minutes. Results Statistic data indicated that change of NF68 was not obvious at 1 day after spinal cord injury, obviously decreased at 3 days, reached the lowest value between 7 to 21 days, and basically reached the control level at 28 days. The changes of the number of glial cells were not obvious at 1, 3 and 7 days compared with the normal level, but increased significantly at 14 days. It increased with time and reached peak at 28 days. Conclusions The similarity of NF changes between spinal cord injury and traumatic brain injury shows there is probable diffuse axonal injury in spinal cord injury. And the glial cells growth is probably helpful for the repair of spinal cord injury.