| Abstract: | Activity of lactate dehydrogenase (LD) and its isozymes in the injured zone and in adjacent areas of the skin was investigated at various times after infliction of burns in rats. On the 1st–8th day after burning total LD activity was reduced in the zone of the scab and in the underlying tissues by 70–80% and in the border and intact skin by 50%. These changes were accompanied by changes in the LD isozyme spectrum in the injured tissue: On the 1st day the activity of fraction 5 was sharply increased and activity of fractions 2 and 3 reduced; by the 8th day, on the other hand, some decrease in activity of fraction 5 and an increase in activity of fractions 2 and 3 were observed. By the 14th–22nd day after burning total LD activity remained low, the LD isozyme spectrum in the border and scab was largely restored to normal, whereas in the underlying tissue changes in the relative proportions of LD isozymes were still present (a decrease in activity of fractions 1–3 and an increase in activity of fraction 5).Laboratory of Biochemistry, A. V. Vishnevskii Institute of Surgery, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR M. I. Kuzin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 9, pp. 294–297, September, 1977. |
