| 大豆皂甙AI对心肌作用的单钙通道分析及ESR谱研究 |
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| 引用本文： | 曾庆华,孙晓霞,孙成文,战术,钟国赣.大豆皂甙AI对心肌作用的单钙通道分析及ESR谱研究[J].吉林大学学报(医学版),1997(4). |
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| 作者姓名： | 曾庆华 孙晓霞 孙成文 战术 钟国赣 |
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| 作者单位： | 基础医学院生理教研室 |
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| 摘 要： | 利用细胞贴附式膜片钳技术，在Ｗｉｓｔａｒ大鼠单个心室肌细胞上记录了Ｔ、Ｌ、Ｂ型钙通道的单通道活动，大鼠皂甙单体ＡＩ２０μｍｏｌ／Ｌ显著抑制三型钙通道的活动，使其开放概率减少，开放时间缩短，对通过通道的离子流幅值无明显影响，用电子自旋共振法测定了培养心肌细胞的自由基含量。大豆皂甙单体ＡＩ２μｇ／ｍｌ显著抵消黄嘌呤０．４２ｍｍｏｌ／Ｌ－黄嘌呤氧化酶５．３ｎｍｏｌ／Ｌ所致的自由基含量增多。
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| 关 键 词： | 钙通道 大豆皂甙 膜片钳 电子自旋共振 |
Single channel analysis and ESR spectral study on the myocardical effects of Soyasaponin AI |
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| Zeng Qinghua,Sun Xiaoxia,Sun Chengwen,et al.Single channel analysis and ESR spectral study on the myocardical effects of Soyasaponin AI[J].Journal of Jilin University: Med Ed,1997(4). |
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| Authors: | Zeng Qinghua Sun Xiaoxia Sun Chengwen |
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| Institution: | Department of Physiology |
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| Abstract: | Single channel activity of type T,L,B Ca 2+ channels were recorded on single ventricular myocardiocytes of Wistar rats,using cell attached configuration of patch clamp technique.Soyasaponin monomer AI 20 μmol/L inhibited the activities of the three types of Ca 2+ channel significantly.The open state propability was decreased and the open time was shorened.2 μg/ml significantly antagonized the increase of the free radical content induced by xanthine 0.42 mmol/L and Xanthic oxidase 5.3 nmo/L |
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| Keywords: | Ca 2+ channel Soyasaponin Patch clamp Electron spin resonance |
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