| 转染P21WAF1基因对BT—325细胞端粒酶表达的影响 |
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| 引用本文： | 张传山,王文亮,王占祥,胡沛臻,张衍国,马福成. 转染P21WAF1基因对BT—325细胞端粒酶表达的影响[J]. 西北国防医学杂志, 2001, 22(1): 24-26 |
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| 作者姓名： | 张传山 王文亮 王占祥 胡沛臻 张衍国 马福成 |
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| 作者单位： | 1. 第四军医大学附属西京医院:病理科,
2. 第四军医大学附属西京医院:神经外科, |
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| 摘 要： | 目的：观察外源性p231WAF1基因对BT－325细胞的作用，探讨p21WAF1表达水平的改变对细胞端粒酶活性的影响。方法：经脂质体介导的方法将PCEP－WAF1质粒导入BT－325细胞中，细胞克隆转移扩大培养后，采用TRAP－PCR－ELISA、TUNEL和电镜等技术检测外源性p21WAF1基因对BT－325细胞端粒活性和细胞凋亡的作用。结果：较对照组相比，转染外源性p21WAF1基因的BT－325细胞端粒酶表达水平显著下降，伴有显著细胞凋亡现象。结论：外源性p21WAF1基因的表达可促进BT－325细胞端粒酶活性降低，诱导细胞显著凋亡，提示端粒酶的活性表达可能与细胞周期调控存在密切联系。
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| 关 键 词： | 星形细胞瘤 端粒酶 P^21WAF1 细胞凋亡 基因转染 BT－325细胞 |
| 文章编号： | 1007-8622(2001)01-0024-03 |
| 修稿时间： | 2000-06-26 |
Effect on telomeraseactivity of BT |
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| ZHANG Chuan-shan ,WANG Wen-liang ,WANG Zhan-xiang ,et al.. Effect on telomeraseactivity of BT[J]. Medical Journal of National Defending Forces in Northwest China, 2001, 22(1): 24-26 |
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| Authors: | ZHANG Chuan-shan WANG Wen-liang WANG Zhan-xiang et al. |
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| Affiliation: | ZHANG Chuan-shan 1,WANG Wen-liang 1,WANG Zhan-xiang 2,et al . |
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| Abstract: | Objective:To observe the effect of exogenous p21 WAF1 expression on BT-325 cells and to investigate p21 WAF1 expression on cellular apoptosis and telomerase activity.Methods:[WT5BZ]PCEP-WAF1(p21 WAF1 cDNA gene eucaryotic expression vector) was introduced into BT-325 cells by using lipofectin mediation;TUNEL and TRAP in combination of ELISA were used to determine BT-325/PCEP-WAF1 apoptosis and telomerase activity after cloning. Results:Compared with control,BT-325/PCEP-WAF1 presented a significant drop in telomerase activity expression,accompanied by remarkable apoptosis.Exogenous p21 WAF1 over-expression could inhibit cell proliferation.Conclusion:Exogenous p21 WAF1 expression promotes apoptosis of BT-325 cells and reduces telomerase activity and can inhibit cell proliferation,resulting in a delay cell cycle. |
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| Keywords: | Astrocytoma Telomerase p21 WAF1 Apoptosis |
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