肝细胞癌组织中乙型肝炎病毒和丙型肝炎病毒的检测

目的检测肝细胞癌（hepatocellular carcinoma，HCC）组织中乙型病毒性肝炎（virus hepatitis type B）表面抗原（HB—sAg）和丙型病毒性肝炎（virus hepatitis type C）核心抗原（HCVAg）的表达。方法应用免疫组织化学法检测78例HCC及癌旁肝组织HBsAg和HCVAg的表达。同时比较患者的HBsAg和HCVAg血清学检测结果。结果HCC及癌旁肝组织中HBsAg和HCVAg阳性及HBsAg和HCVAg双重阳性表达率分别为9.1％、6．4％、2．6％和80．8％、44．9％、29．5％。HCC与癌旁肝组织HBsAg和HCVAg阳性及HBsAg和HCVAg双阳性表达率相互比较有非常显著性差异（P〈0．01）。合并肝硬化组和无肝硬HCC组中HCC与癌旁肝组织HBsAg和HCVAg阳性、HBsAg及HCVAg双阳性表达率相互比较也有非常显著性差异（P〈0．01）。HCC的HBsAg和HCVAg血清学检测的结果与手术标本HBsAg和HCVAg免疫组织化学检测的结果相吻合。结论HCC的发生与HBV和HCV的感染密切相关。

Detection of Hepatitis B and C Virus in Hepatocellular Carcinoma

Objective To detect the expression of HBsAg and HCVAg in hepatocellular carcinoma(HCC).Methods Immunohistochemical method was used to detect HBsAg and HCVAg protein in carcinoma tissue,paracarcinoam hepatic tissue,and serum specimens of 78 cases of HCC for comparison study.Results The positive expression rates of HBsAg,HCVAg,both HBsAg and HCVAg protein were 9.1%,6.4% and 2.6% respectively in carcinoma tissue,and 80.8%,44.9% and 29.5% respectively in paracarcinoma tissue,showing a significant difference between the carcinoma tissue and paracarcinoma hepatic tissue(P<0.01).The positive expression rates of HBsAg,HCVAg,both HBsAg and HCVAg Protein between carcinoma tissue and paracarcinoma hepatic tissue were also significantly different in HCC with cirrhosis or without(P<0.01).Serological detection of HBsAg and HCVAg had the same result as tissue detection by immunohistochemistry.Conclusion HBV and HCV are closely related to the carcinogenesis of HCC.Multi-factors may play roles in the long process of hepatocarcinogenesis.