艾灸对脾虚大鼠小肠运动吸收功能及ATP含量的影响

目的:探讨艾灸温补脾胃的作用机制.方法:40只SD大鼠随机分为4组:空白对照组(A组),脾虚模型组(B组),艾灸穴位组(C组),中药对照组(D组).采用200％大黄水浸剂灌胃造成脾虚大鼠模型,C组艾灸“足三里”“中脘”“关元”“脾俞”“胃俞”等穴,D组用中药四君子汤灌胃治疗,A组与B组不予治疗干预.观察大鼠一般症状,记录小肠推进率,间苯三酚法测定血清D-木糖含量,比色法测定空肠组织三磷酸腺苷(ATP)水平.结果:与A组比较,B组大鼠脾虚一般症状积分增高(P＜0.01),小肠推进率、血清D-木糖含量以及空肠组织ATP含量均降低(P＜0.05,P＜0.01);与B组比较,C组和D组大鼠脾虚一般症状积分降低(均P＜0.01),小肠推进率、血清D-木糖含量以及空肠组织ATP含量均增高(P＜0.05,P＜0.01);C、D两组比较差异无统计学意义(P＞0.05).结论:艾灸“足三里”等穴位可改善大鼠脾虚症状,增进小肠运动吸收功能,改善小肠能量代谢,与中药四君子汤疗效相当.

Effect of moxibustion on motility, absorption and content of ATP in small intestine of spleen-deficiency rats

Objective To explore the mechanism of reinforcing function of moxibustion to spleen-stomach.Methods Forty healthy Sprague Dawley rats were randomly divided into 4 groups: group A(blank group),group B(model group),group C(moxibustion group) and group D(herbs group).The rat model of spleen-deficiency was established by intragastric administration with 200% Dahuang(Rhubarb) infusion.The rats in group A and B,and D served as the blank control,model,and Sijunzi decoction group respectively,while those in group C received moxibustion at "Zusanli"(ST 36),"Zhongwan"(CV 12),"Guanyuan"(CV 4),"Pishu"(BL 20) and "Weishu"(BL 21),etc.The common symptoms and intestinal propulsive rate were observed.The content of D-xylose in serum was detected by phloroglucinol method.Colorimetry method was used to detected content of ATP in jejunum tissues.Results Compared with group A,the symptom score in group B was increased significantly(both P<0.01),while the intestinal propulsive rates,the content of D-xylose in serum and ATP in jejunum tissues were decreased significantly(P<0.05,P<0.01).Compared with group B,the symptom score in group C and D was decreased significantly(both P<0.01),while the intestinal propulsive rates,the content of D-xylose in serum and ATP in jejunum tissues were increased significantly(P<0.05,P<0.01).There were no significant difference between group C and D(P>0.05).Conclusion Moxibustion at "Zusanli"(ST 36) etc.could relieve symptoms of spleen-deficiency,enhance motility and absorption functions of small intestine and improve metabolism of small intestine.The efficacy is equal to administration of Sijunzi decoction.