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| Experimental Study on Meiotic Spindles and Chromosomes of Mouse Mature （MⅡ） Stage Oocytes under Laser Scanning Confocal Microscopy | |
| 引用本文： | 王雁林 朱桂金 李霞 李小兰. Experimental Study on Meiotic Spindles and Chromosomes of Mouse Mature （MⅡ） Stage Oocytes under Laser Scanning Confocal Microscopy[J]. 华中科技大学学报(医学英德文版), 2006, 26(3): 353-355. DOI: 10.1007/BF02829574 |
| 作者姓名： | 王雁林 朱桂金 李霞 李小兰 |
| 作者单位： | Reproductive Medical Center Department of Obstetrics and Gynecology，Reproductive Medical Center Department of Obstetrics and Gynecology，Department of Surgery Public Health Clinic of Dudian Town Binzhou 256657 China，Molecule Medical Center Tongji Hopital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China |
| 摘 要： | The in vitro culture,maturation and cryopr-eservation of oocytes are all the i mportant parts inthe assisted reproductive technology.Mature oo-cyte(i.e.MⅡoocytes)is arrested at the meta-phase stage of meiosisⅡ,in which chromosomesare attached to the spindle microtubles and line uponthe equatorial plateinthe normal spindles.Dis-organized structure of spindles can not onlylead tosuch morphologic changes as unwound chromo-somes but also the chromosome nondisjuction andresultant aneuploid.When t… |
| 关 键 词： | 染色体 卵母细胞 显微镜 检查 |
| 收稿时间： | 2006-02-19 |
Experimental study on meiotic spindles and chromosomes of mouse mature (M II) stage oocytes under laser canning confocal microscopy |
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| Wang Yanlin,Zhu Guijin,Li Xia,Li Xiaolan. Experimental study on meiotic spindles and chromosomes of mouse mature (M II) stage oocytes under laser canning confocal microscopy[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2006, 26(3): 353-355. DOI: 10.1007/BF02829574 | |
| Authors: | Wang Yanlin Zhu Guijin Li Xia Li Xiaolan |
| Affiliation: | (1) Reproductive Medical Center, Department of Obstetrics and Gynecology, Tongji Hopital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China;(2) Molecular Medical Center, Tongji Hopital, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China;(3) Department of Surgery, Public Health Clinic of Dudian Town, 256657 Binzhou, China |
| Abstract: | Summary Taking the mouse as a model, the experimental method of observing the morphology of meiotic spindles and chromosomes in mature oocytes were investigated in order to evaluate the effects of various interventions on the quality of oocytes accurately and rapidly. Laser scanning confocal microscope (LSCM) was used to examine the meiotic spindles and chromosomes by the technologies of optical section and three-dimensional (3D) image reconstruction. The results showed that the configurations of meiotic spindles and chromosomes could be observed clearly by LSCM. The normal rate of meiotic spindles and chromosomes was 82% and 86% respectively. It was concluded that the LSCM was a valid instrument to observe the meiotic spindles and chromosomes of mature oocytes and could be used as a valid method to evaluate the quality of M II oocytes. WANG Yanlin, female, born in 1972, M. D., Ph. D., Doctor in Charge |
| Keywords: | laser scanning fonfocal microscope oocytes spindle chromosome |
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