| Trappin-2对HaCaT细胞及银屑病跨膜模型增殖的抑制作用 |
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| 引用本文： | 阎衡,翟志芳,周春丽,钟华,郝飞. Trappin-2对HaCaT细胞及银屑病跨膜模型增殖的抑制作用[J]. 第三军医大学学报, 2008, 30(8): 746-749 |
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| 作者姓名： | 阎衡 翟志芳 周春丽 钟华 郝飞 |
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| 作者单位： | 第三军医大学西南医院皮肤科,重庆市皮肤性病研究所,重庆,400038;第三军医大学西南医院皮肤科,重庆市皮肤性病研究所,重庆,400038;第三军医大学西南医院皮肤科,重庆市皮肤性病研究所,重庆,400038;第三军医大学西南医院皮肤科,重庆市皮肤性病研究所,重庆,400038;第三军医大学西南医院皮肤科,重庆市皮肤性病研究所,重庆,400038 |
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| 摘 要： | 目的 构建Trappin-2的真核表达体系,初步探讨Trappin-2对HaCaT细胞及银屑病跨膜模型增殖的影响.方法 采用DNA重组技术构建Trappin-2的表达载体pIRES2-EGFP-Trappin-2,通过脂质体法转染入宫颈癌HeLa细胞.将Trappin-2高表达上清加入HaCaT细胞及银屑病跨膜模型中,通过3H-TdR掺入法、MTT法、流式细胞术及组化染色Ki67检测对增殖的影响.结果 Trappin-2作用后MTY和cpm值变化率显示Trappin-2可抑制HaCaT细胞的代谢与DNA合成,同时可使HaCaT细胞G2 S期细胞比例下降,而G1期细胞比例增加.Trappin-2可下调银屑病皮损Ki67表达水平.结论 成功将Trappin-2基因转染HeLa细胞,并进行有效表达,初步证实Trappin-2具有抑制HaCaT细胞及银屑病跨膜模型增殖的特性.
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| 关 键 词： | Trappin-2 真核表达 HaCaT细胞 器官培养 增殖 |
| 文章编号： | 1000-5404(2008)08-0746-04 |
| 修稿时间： | 2007-07-10 |
Trappin-2 inhibits the proliferation of HaCaT cells and transwell psoriatic skin organ culture models |
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| YAN Heng,ZHAI Zhi-fang,ZHOU Chun-li,ZHONG Hua,HAO Fei. Trappin-2 inhibits the proliferation of HaCaT cells and transwell psoriatic skin organ culture models[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(8): 746-749 |
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| Authors: | YAN Heng ZHAI Zhi-fang ZHOU Chun-li ZHONG Hua HAO Fei |
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| Affiliation: | YAN Heng,ZHAI Zhi-fang,ZHOU Chun-li,ZHONG Hua,HAO Fei(Department of Dermatology , Venereology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To construct a eukaryotic expression vector of Trappin-2 gene and investigate its effect on the proliferation of HaCaT cells and transwell psoriatic skin organ culture models.Methods The pIRES2-EGFP-Trappin-2 expression vector was constructed by DNA recombinant technique and transfected into HeLa cells with lipofectamineTM2000,then the supernatant containing Trappin-2 was added to HaCaT cells and transwell psoriatic skin organ culture models.3H-TdR incorporation,MTT method,cell cycle analysis and ... |
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| Keywords: | Trappin-2 |
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