|
|||||
|
|
| Expression of nitric oxide synthase in T-cell-dependent liver injury initiated by ConA in Kunming mice | |
| 引用本文： | 张修礼 曲建慧 万谟彬 权启镇 孙自勤 王要军 江学良 李文波. Expression of nitric oxide synthase in T-cell-dependent liver injury initiated by ConA in Kunming mice[J]. 中国人民解放军军医大学学报, 2004, 19(2): 112-114 |
| 作者姓名： | 张修礼 曲建慧 万谟彬 权启镇 孙自勤 王要军 江学良 李文波 |
| 作者单位： | [1]DepartmentofGastroenterology,GeneralHospital,PLAJi＇nanMilitaryAreaCommand,Ji＇nan250031,China [2]DepartmentofInfectiousDiseases,No.106HospitalofPLA,Ji＇nan250022,China [3]DepartmentofInfectiousDiseases,ChanghaiHospital,SecondMilitaryMedicalUniversity,Shanghai200433,China |
| 摘 要： | To investigate whether nitric oxide synthase (NOS) is expressed in T-cell-dependent liver injury initiated by concanavalin A (ConA) in Kunming mice and study the possible effect of nitric oxide(NO) on liver injury models. Methods: Liver injury in Kunming mice was induced by administration of ConA through tail vein. Expression of NOS inthe liver was detected by NADPH diaphorase staining method. The possible effect of NO on liver injury models was obtainedby L-NAME injection to suppress synthesis of NO. Results: NOS has a strong expression in hepatocytes after ConA injection, especially in those close to the central vein, while only a weak expression was found in the epithelial cells in contrml group. Liver injury became more serious when NO symhesis was inhibited by L-NAME, accompanied by great malondlalde-hyde(MDA) increase in serum and severe intrahepatic vascular thrombosis. Conclusion: NOS markedly expressed in ConAinduced liver injury, which may subsequently promote nitric oxide synthesis. Increasement of nitric oxide has a protective ef-fect on ConA-induced liver injury. |
| 关 键 词： | 一氧化氮合酶 T细胞相关性肝损伤 动物模型 刀豆体球蛋白A 病理学 |
Expression of nitric oxide synthase in T-cell-dependent liver injury initiated by ConA in Kunming mice |
|
| ZHANG Xiu-li ,QU Jian-hui ,WAN Mo-bin ,QUAN Qi-zhen ,SUN Zi-qin ,WANG Yao-jun ,JIANG Xue-liang ,LI Wen-bo. Expression of nitric oxide synthase in T-cell-dependent liver injury initiated by ConA in Kunming mice[J]. Journal of Medical Colleges of PLA(China), 2004, 19(2): 112-114 | |
| Authors: | ZHANG Xiu-li QU Jian-hui WAN Mo-bin QUAN Qi-zhen SUN Zi-qin WANG Yao-jun JIANG Xue-liang LI Wen-bo |
| Affiliation: | ZHANG Xiu-li 1*,QU Jian-hui 2,WAN Mo-bin 3,QUAN Qi-zhen 1,SUN Zi-qin 1,WANG Yao-jun 1,JIANG Xue-liang 1,LI Wen-bo 1 1Department of Gastroenterology,General Hospital,PLA Ji'nan Military Area Command,Ji'nan 250031,China 2Department of Infectious Diseases,No. 106 Hospital of PLA,Ji'nan 250022,China 3Department of Infectious Diseases,Changhai Hospital,Second Military Medical University,Shanghai 200433,China |
| Abstract: | Objective: To investigate whether nitric oxide synthase (NOS) is expressed in T-cell-dependent liver injury initiated by concanavalin A (ConA) in Kunming mice and study the possible effect of nitric oxide(NO) on liver injury models. Methods: Liver injury in Kunming mice was induced by administration of ConA through tail vein. Expression of NOS in the liver was detected by NADPH diaphorase staining method. The possible effect of NO on liver injury models was obtained by L-NAME injection to suppress synthesis of NO. Results: NOS has a strong expression in hepatocytes after ConA injection, especially in those close to the central vein, while only a weak expression was found in the epithelial cells in control group. Liver injury became more serious when NO synthesis was inhibited by L-NAME, accompanied by great malondialdehyde(MDA) increase in serum and severe intrahepatic vascular thrombosis. Conclusion: NOS markedly expressed in ConA-induced liver injury, which may subsequently promote nitric oxide synthesis. Increasement of nitric oxide has a protective effect on ConA-induced liver injury. |
| Keywords: | nitric oxide synthase nitric oxide concanavalin A liver injury |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |