瘢痕疙瘩家系永生淋巴细胞株的建立及其染色体分析

目的建立瘢痕疙瘩家系永生淋巴细胞株,并对该细胞株进行染色体核型分析,探讨用这种方法建立瘢痕疙瘩永生细胞库的可行性和可靠性。方法采用EB病毒转化技术,把瘢痕疙瘩家系外周血B淋巴细胞转化成永生化淋巴母细胞系(LCL)。分别对建株前和建株后10、20、30、35代的细胞株进行染色体核型分析。结果成功地对瘢痕疙瘩家系27个个体建立了永生细胞株,建株后10代、20代的细胞株染色体未发现明显异常;而培养30代、35代的细胞株染色体发生数目和结构的异常。结论瘢痕疙瘩家系永生淋巴细胞株可以为以后的研究提供随时可取的实验材料,在用做遗传学分析时宜在转化早期进行。

Establishment of immortalized B-lymphoblastoid cell lines of keloid pedigree and its karyotype analysis

Objective To establish immortalized B-lymphoblastoid cell lines of keloid pedigree transformed with Epstein-Barr(EB)virus and conduct karyotype analysis of the cells.Methods Immortalized B-lymphoblastoid cell lines were established by EB virus transformation of the peripheral blood B lymphocytes from the members of keloid pedigree.Karyotype analysis was performed for the cultured cells of passages 10,20,30,and 35 to evaluate their genetic stability.Results Altogether 27 immortalized lymphoblastoid cell lines with stable chromosome were obtained successfully from the keloid pedigree.No chromosomal abnormalities were found in the cultured cells until passages 30 and 35,in which variation in chromosome number and structure are detected.Conclusion The cell lines of the keloid pedigree established in this studsy can be useful in future studies,and genetic analysis is conducted preferably with cells of early passages.