5种温热性活血化瘀中药对寒凝血瘀证大鼠内分泌激素及5-羟色胺、去甲肾上腺素的影响

目的探讨温热性活血化瘀中药干预寒凝血瘀证模型大鼠的性-效机制。方法实验动物为远交群SD大鼠,采用冰水浸泡法复制寒凝血瘀证模型。300只造模成功的寒凝血瘀证模型大鼠,按体重均衡原则随机分为5组,每组60只,分别用于平行观察三碘甲腺原氨酸（T3）、四碘甲腺原氨酸（T4）、孕酮（P）、5-羟色胺（5-HT）及去甲肾上腺素（NE）5个指标;各组再分为模型组、姜黄组、川芎组、红花组、延胡索组、乳香组6个亚组,每组10只;每个指标组分别设正常对照组,每组10只,共使用正常大鼠50只。完成1项观察指标需70只大鼠（7组）,5个观察指标共用350只大鼠。各给药组按20 g生药/kg体重灌胃给予相应的中药水煎液,1天1次,1次3 m L,连续7天;同时正常组和模型组予等量生理盐水。检测各组大鼠给药前、给药1周T3、T4、P、5-HT及NE的含量。结果与本组给药前比较,给药后川芎组T3、乳香组T3、各给药组T4、川芎组5-HT、各给药组NE及各给药组P水平均升高（P〈0.05）;与正常组比较,模型组T3、T4、5-HT、NE及P水平均降低（P〈0.05）。与模型组比较,各给药组T3、T4、5-HT、NE水平升高（P〈0.05）,P水平川芎组、乳香组差异有统计学意义（P〈0.05）。结论温热性活血化瘀中药对寒凝血瘀证模型大鼠血清T3、T4、NE及P等具有组群特征性调控作用,可促进机体甲状腺-性腺轴功能,增强内分泌系统功能,这可能是温热性活血化瘀中药调治寒性血瘀证的药效学机制之一。

Effect of 5 Warm-Hot Nature Chinese Drugs for Promoting Blood Circulation and Removing Blood Stasis on 5-HT,NE,and Endocrine Hormones of Rats of Cold Coagulation and Blood Stasis Syndrome

Objective To study the mechanism of warm-hot nature Chinese drugs（ WHNCD） for promoting blood circulation and removing blood stasis（ PBCRBS） for intervening model rats of cold coagulation and blood stasis syndrome（ CCBSS）. Methods CCBSS rat model was set up in outbred SD rats using ice water immersion method. Totally 300 successfully modeled CCBSS rats were randomly divided into 5 groups according to the principle of balance weight,60 in each group. Contents of triothyrone（ T3）,tetraiodothyroine（ T4）,progesterone（ P）,5-hydroxytryptamine（ 5-HT）,and noradrenalin（ NE） were paralleledly detected in all groups. Then rats in each group were subdivided into 6 subgroups as the model group,the curcuma group,the Ligsticum Chuanxiong group,the safflower group,the Rhizoma Corydalis group,and the Olibanumg group. Besides,5 normal control groups were set up for 5 indices,50 rats in total. We need 70 rats（ 7 groups）to finish observing 1 index,350 rats in total for 5 indices. Except those in the model group and the normal control group,rats were administered with corresponding decoction at 20 g crude drugs / kg body weight by gastrogavage,3 m L each time,oncedaily for 7 successive days. Equal volume of normal saline was given to rats in the normal control group and the model group.Contents of T3,T4,P,5-HT,and NE were detected before treatment and 1 week after treatment. Results Compared with before treatment in the same group,T3 increased in the Ligsticum Chuanxiong group and the Olibanumg group,5-HT increased in the Ligsticum Chuanxiong group,T4,NE,and P increased in all medicated groups（ P 〈0. 05）. Compared with the normal control group,contents of T3,T4,5-HT,NE,and P in the model group decreased（ P 〈0. 05）. Compared with the model group,contents of T3,T4,5-HT,and NE increased in each medicated group（ P 〈0. 05）. There was statistical difference in contents of P between the Ligsticum Chuanxiong group and the Olibanumg group（ P 〈0. 05）. Conclusions WHNCD for PBCRBS had