四种食用真菌提取物预防小鼠酒精性氧化损伤的研究

目的 ]评价灵芝、云芝、灰树花、姬松茸 4种食用真菌提取物预防小鼠酒精性氧化损伤的作用。 方法 ]每日灌服 4种食用真菌提取物 ,每鼠各 1 5g/kgbw ,共 3 0d ,结束时阳性对照组及各受试物组一次灌胃 5 0 %乙醇 12ml/kg ,阴性对照组给予 70 %葡萄糖溶液 ,禁食 12h后处死。检测血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)和谷胱甘肽s 转移酶 (GST)、肝匀浆丙二醛 (MDA)、还原型谷胱甘肽 (GSH)、谷胱甘肽过氧化物酶 (GSH Px)、超氧化物歧化酶 (SOD)和甘油三酯 (TG)。同时计算肝体比。 结果 ]灵芝提取物组GSH、GSH Px显著升高 (P <0 0 5 ) ,MDA、TG含量明显下降 (P <0 0 5 )。结论 ]给予小鼠相同剂量的 4种真菌提取物 ,其中灵芝提取物有较好的预防酒精性氧化损伤作用

The Screening Study on the Protective Effect of 4 Kinds of Edible Fungus Extract on Alcoholic Oxidative Injury in Mice

To evaluate the protective effect of 4 kinds of edible fungus extract on alcoholic oxidative injury in mice. 4 kinds of edible fungus extract were given at a dose of 1 5 g/kg bw per mouse through gastric gavaging for 30 days,the control group was given water. 30 days later,positive control group and test groups were gavaged 50% ethanol,negative control group was given 70% glucose at once,both at a dose of 12 ml/kg. The mice were fasted for 12 hours,then killed. The activities of serum ALT,AST,GST and liver GSH Px,SOD,the concentration of liver MDA,GSH,TG,and the ratio of liver to body were examined. It was especially found that ganoderma extract could significantly increase GSH and GSH Px ( P <0 05),decrease MDA and TG ( P <0 05). Conclusion] Among 4 edible fungus extracts,the ganoderma extract has better protective effect on alcoholic oxidative injury in mice.