蝎毒联合ADM对人乳腺癌MCF-7/细胞的凋亡诱导作用及其耐药逆转的研究

目的探讨蝎毒(BMK)联合阿霉素(ADM)对人乳腺癌MCF-7/ADM细胞的凋亡诱导及其耐药逆转作用。方法MTT法、荧光分光光度法、流式细胞术、紫外分光光度术和免疫细胞化学法。结果①非细胞毒性剂量(3.0μg/m l)蝎毒能显著降低MCF-7/ADM的IC50(P<0.01),增加MCF-7/ADM细胞内ADM的药物积累(P<0.01)。②蝎毒(3.0μg/m l)联合ADM后增强了对耐药细胞的凋亡诱导作用,凋亡率由8.9±0.01%上升为12.6±0.21%(P<0.01)。③蝎毒(3.0μg/m l)能够显著降低耐药细胞内谷胱甘肽S转移酶(GSTs)酶的活性(P<0.01)。结论蝎毒能够部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与抑制耐药细胞GSTs活性有关。

Effect of Inducing Apoptosis and Resistance Reversing of BMK Combined with ADM on Human Breast Cancer MCF-7/ADM Cell Line

Objective To study the effect of inducing apoposis and resistance reversing of buthus martensii karsch(BMK) combined with adriamycin(ADM) on human breast cancer MCF-7/ADM cell line.Methods The MTT assay,fluorescent-spectrophotometry,flow cytometry,UV-spectrophotometry and immunocytochemistry were used.Results ①The non-cytotoxic dose of BMK(μg/ml) significantly decreased the IC_(50) value of ADM and increased the intracellular ADM accumulation in MCF-7/ADM cells(P<0.01,P<0.05).②BMK(3μg/ml)combined with ADM increased the apoptosis inducing effect of ADM to MCF-7/ADM cells,which of apoptosis percentage was increased from(8.9±0.01)﹪ to(12.6±0.21)﹪(P<0.01).③BMK(3μg/ml) significantly decreased glutathione-S-transferase(GFTs) activity in MCF-7/ADM cells(P<0.01).Conclusion BMK could partially reverse resistance to ADM in human breast cancer MCF-7/ADM cell line,which may be associated with the decreased GSTs activity.