胃癌和癌旁黏膜与距癌远端切缘胃黏膜基因表达谱差异的研究

目的用基因芯片技术研究胃癌 （T）和癌旁黏膜 （P）与距癌远端切缘胃黏膜 （C）基因表达谱差异,筛选与早期癌变相关的基因.方法利用国际学术界公认的标准化 Affymetrix公司生产的 U133A基因芯片分别检测 T和 P及 C基因表达谱,并利用生物信息学方法对检测结果进行分析.结果 （1）T与 C比较差异 4倍以上共有 766个基因,其中表达上调 [信号比的对数值 （SLR）〉2]有 530个,表达下调 （SLR〈- 2）有 236个;（2）P与 C比较差异 4倍以上共有 64个基因,其中表达上调 （SLR 〉2）有 50个,表达下调 （SLR〈- 2）有 14 个;（3）T和 P同时与 C比较 （T、 P两者之一差异 4倍以上 ）共有 143个相同的基因,其中表达上调 （SLR 〉2）有 108个,表达下调 （SLR〈- 2）有 35个.结论癌旁黏膜在基因表达水平上显示 143个基因与胃癌表达的基因相同,提示这些基因可能与早期胃癌癌变的启动和演化有关.

Study on gene expression profile difference in gastric cancer,pericancerous mucosa and normal gastric mucosa from the distant cutting margin by oligonucleotide microarray

Objective To study the difference of gene expression profiles in gastric cancer (T),pericancerous mucosa (P) and the gastric mucosa from distant cutting margin (C),and to screen an associated novel gene in early gastric carcinogenesis by oligonucleotide microarray. Methods U133A (Affymetrix,Santa Clara,CA) gene chip was used to detect the gene expression profile difference in T,P and C,respectively. Bioinformatics was used to analyze the detected results. Results When gastric cancer was compared with normal gastric mucosa,766 genes were found,with a difference of more than four times in expression levels,including 530 up-regulated [Signal Log Ratio (SLR) >2],and 236 down-regulated (SLR<-2). When P was compared with C,64 genes were found,with a difference of more than four times in expression levels,including 50 up-regulated (SLR >2),and 14 down-regulated (SLR<-2). Compared with C,a total of 143 genes with a difference of more than four times in expression levels both in T and P tissues. Of the 143 genes,108 were up-regulated (SLR >2),and 35 were down-regulated (SLR<-2). Conclusions Gene chip can reveal 143 same genes both in pericancerous mucosa and gastric mucosa. These genes may be related to the carcinogenesis and development of early gastric cancer.