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间接免疫荧光法检测解脲支原体
引用本文:陈志和,罗迪青,赖小敏. 间接免疫荧光法检测解脲支原体[J]. 中国优生与遗传杂志, 2001, 9(4): 103-103,107
作者姓名:陈志和  罗迪青  赖小敏
作者单位:1. 广州市第二人民医院皮肤科,510150
2. 广州市第二人民医院内科
3. 中山医科大学微生物教研室
摘    要:目的;探讨间接免疫荧光法(IIF)检测临床标本中解脲支原体的敏感性及特异性.方法用IIF检测了临床培养分离的50例样本,并分别用IIF及培养法对71例临床标本进行检测并比较了两种方法.结果IIF对50例临床分离标本的检出率为100%,而对临床标本的特异性是92.3%,敏感性为75%,但IIF与培养法检测比较没有统计学差异(P>0.10).结论可以用间接免疫荧光法代替培养法检测解脲支原体.

关 键 词:间接免疫荧光法 检测 解脲支原体 IIF 泌尿生殖系感染

Detecting Ureaplama urealyticum by indirect immunofiuorescence
Chen Zhihe,Luo Diqing,Lai Xiaomin.. Detecting Ureaplama urealyticum by indirect immunofiuorescence[J]. Chinese Journal of Birth Health & Heredity, 2001, 9(4): 103-103,107
Authors:Chen Zhihe  Luo Diqing  Lai Xiaomin.
Abstract:Objective:To study the sencitivity and specificity of indirect immunofluorescence(IIF)in detecting ureaplasma urealyticum(UU).50 UU samples from culture were detected by IIF.71 specimens from clinic were examined by both IIF and cultivition.We also compared IIF with cultivition.The Result showed that the sencitivity of IIF was 100% in culture samples and 75% in clinic samples,there was no statistical significant between two methods(P>0.10).The specificity of IIF was 92.3% in clinic samples.It suggested that cultivition could be repalced by IIF in clinic in detecting UU.
Keywords:Indirect immunofluorescence  Detecting  Ureaplasma Urealyticum.
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