前列腺素E1对1-甲脲乙醇酸酐所致肾小管上皮细胞损害的保护作用

目的]研究前列腺素E1 (PGE1)对1-甲脲乙醇酸酐所致肾小管上皮细胞凋亡的保护作用.方法]人近端肾小管上皮细胞(HK2)分三组培养,A组正常对照组；B组1-甲脲乙醇酸酐组:培养基中加入0.5 mmol/L1-甲脲乙醇酸酐；C组PGE1+1-甲脲乙醇酸酐组:培养基中加入0.5 mmol/L 1-甲脲乙醇酸酐和PGE12μg/L.培养48 h后用MTT比色法测光密度(OD)值,并检测培养液NAG酶(N-乙酰-p氨基葡萄糖苷酶)活性；用Annexin V-FITC/PI流式细胞仪检测胞凋亡.结果]B组HK-2细胞OD值较A组显著下降,NAG酶活性上升；C组较B组OD值显著上升而NAG酶活性下降；B组HK-2细胞凋亡率显著高于对照组,C组的凋亡率较B组显著下降,其差异均有统计学意义(P＜0.01).结论]1-甲脲乙醇酸酐有促进肾小管上皮细胞凋亡的作用.PGE1对1-甲脲乙醇酸酐所致肾小管上皮细胞凋亡有一定的保护作用.

Protective Effect of Prostaglandin E1 Against 1-Methylhydantoi Induced HK-2 Cell Apoptosis

Objective] To investigate the protective effect of prostaglandin E1 （PGE1） against 1-mthylhydantoi induced renal tubular epithelial cell apoptosis. Methods]Human distal renal tubular epithelial cells（HK-2） were divided into 3 groups to be cultured. Group A was normal control group. Group ]3（1-methylhydantoi group） was 0.5mmol /L 1-methylhydantoi in the medium. Group C（PGE~ ＋ 1-methylhydantoi group） was 0.5 mmol /L 1- mthylhydantoine and 2l~g/L PGE1 in the medium. MTT method was used to detect optical density（OD） value and the activity of NAG enzyme in culture 48h after culture. Annexin V-FITC/PI flow cytometry was used to detect cell apoptosis. Results] Compared with group A, OD value of HK-2 cells in group B significantly decreased, while NAG increased. Compare with group B, OD value in group C markedly increased, while NAG decreased. HK-2 cell apoptosis in group B was markedly higher than that in control group. Compared with group B, HK-2 cell apoptosis in group C markedly decreased. There were significant differences（ P d0. 01）. Conclusion]l- Methylhydantoi can promote renal tubular epithelial cell apoptosis. PGE1 can protect renal tubular epithelial cell apoptosis induced by 1-methylhydantoi.