ELISA法与2种GICA法检测丙型肝炎病毒抗体结果分析

目的分析探讨ELISA法(酶联免疫吸附试验)和GICA法(胶体金免疫层析试验)两种试纸条检测抗-HCV阳性标本的结果符合率及GICA法检出率较低所产生的原因,以对检测方法和试剂作出正确的选择。方法采用ELISA法和GICA法两种试纸条对134份抗-HCV阳性标本进行检测,并将所测结果进行比较。结果 ELISA、GICA法(英科)、GICA法(爱德)阳性率分别为96.3%、93.3%、76.9%,以ELISA法为标准做统计,GICA法(英科)检测结果差异无统计学意义(P0.05),GICA法(爱德)检测结果差异有统计学意义(P0.01),两种试纸检测的灵敏度及符合率分别为GICA法(英科)为96.9%、97.0%,GICA法(爱德)为79.8%、80.6%,两种试纸条的特异性均为100%。结论 (1)GICA法灵敏度和符合率均较ELISA法低,而不同厂家生产的GICA法试纸条其敏感度与准确度也存在着较大的差异,检测抗-HCV阳性标本时存在一定的漏检,CICA法仅能作为筛查试验或辅助ELISA法复检阳性标本。(2)在考虑单次成本及阳性检出率的前提下,ELISA法仍为抗-HCV检测的首选方法。

ELISA method with two kinds of test paper GICA serum hepatitis C virus antibody results and analysis

Objective analysis of ELISA method（enzyme-linked immunosorbent assay） and GICA Act（colloidal gold immunochromatography assay） test strip for detection of two anti-HCV positive samples results are consistent with the detection rate and low rate of GICA method produced reasons,to the detection methods and reagents to make the right choice.Methods ELISA,and two strips on GICA law 134 anti-HCV positive samples were detected and measured results were compared.Results ELISA,GICA Act（British Branch）,GICA Act（Ed）-positive rates were 96.3%,93.3%,76.9%to the standard ELISA method to do statistics,GICA Act（British Branch） test showed no significant difference（P0.05）,GICA Law（Ed） test results are significantly different（P0.01）,two kinds of test paper detection sensitivity and compliance were GICA Act（British subjects） was 96.9%,97.0%,GICA law（Ed） was 79.8%,80.6%,specificity of two strips of 100%.Conclusion（1） GICA method is sensitive and consistent with rates lower than the ELISA method,but different manufacturers GICA assay sensitivity and accuracy of paper there are large differences in detection of anti-HCV positive samples were missed when there is a certain,CICA method only as a screening test or ELISA,or review the positive samples auxiliary.（2） In considering the cost and single-positive rate of the premise,ELISA anti-HCV detection method is still the preferred method