野木瓜果实总皂苷体外抗肿瘤作用研究

目的 研究野木瓜果实总皂苷对人肺癌细胞A549、人肝癌细胞BEL-7402和人胃癌细胞SGC-7901增殖的影响.方法 将生长状态良好的A549细胞、BEL-7402细胞和SGC-7901细胞分为7组:阴性对照组,野木瓜果实总皂苷组(40、80、160、320、640 mg·L-1)组和丝裂霉素组(10 mg·L-1),药物作用12、24、48 h后,MTT法测定细胞增殖抑制率.结果 野木瓜果实总皂苷能显著抑制A549细胞、BEL-7402细胞和SGC-7901细胞的增殖,呈一定剂量和时间依赖关系,作用A549细胞12、24、48 h的半数抑制浓度(IC50)分别为492.38、293.43、141.84 mg·L-1;作用BEL-7402细胞12、24、48 h的IC50分别为427.74、243.91、101.79 mg·L-1;作用SGC-7901细胞12、24、48 h的IC50分别为463.35、256.77、113.22 mg·L-1.结论 野木瓜果实总皂苷有明显抑制A549细胞、BEL-7402细胞和SGC-7901细胞体外增殖的作用,其中BEL-7402细胞对野木瓜果实总皂苷最为敏感.

Anti-tumor effect of total saponins from the fruit of Stauntonvine chinensis DC in vitroZHU Xiaobo,LI Yuting,ZHU Xinting,LIU Yun,LI Dayu,FAN Fang,LI Changfu (Department of Biochemistry,Zunyi Medical College,Zunyi,Guizhou 563000,China) Abstract:Objective

Objective To investigate the inhibitory effect of total saponins from the fruit of Stauntonia chinensis DC on human lung cancer cell (A549),human hepatocellular cell (BEL-7402) and human gastric cancer cell (SGC-7901) in vitro.Methods The A549 cells,BEL-7402 cells and SGC-7901 cells were randomized into seven groups: negative control group,total saponins (40,80,160,320,640 mg·L-1) groups and mitomycin (10 mg·L-1) group.The proliferation inhibition rate in each group was calculated by MTT after incubated with drugs for 12,24,48 h.Results The total saponins from the fruit of Stauntonia chinensis DC could significantly inhibit the proliferation of A549 cells,BEL-7402 cells and SGC-7901 cells were in dose-and time-dependent.A549 cells were incubated for 12,24,48 h by total saponins,IC50 were 492.38,293.43,141.84 mg·L-1 respectively.BEL-7402 cells were incubated for 12,24,48 h by total saponins,IC50 were 427.74,243.91,101.79 mg·L-1 respectively.SGC-7901 cells were incubated for 12,24,48 h by total saponins,IC50 were 463.35,256.77,113.22 mg·L-1 respectively.Conclusions The total saponins from the fruit of Stauntonia chinensis DC could obviously inhibit the proliferation of A549 cells,BEL-7402 cells,and SGC-7901 cells.Among them,BEL-7402 cells were the most sensitive to the total saponins.