驻极体诱导3T3细胞内游离钙离子浓度的变化

目的：研究公主体对3T3细胞内游离Ca^2 浓度变化的,体诱导3T3细胞凋亡与游离Ca^2 浓度变化之间的关系。方法：选用－300V和－500V驻极体作用3T3细胞48h，即时依次加入CaCl2,A23187,EDTA，通过流式细胞术检测细胞内Ca^2 浓度的变化和3T3细胞的凋亡量。结果：（1）负极性驻极体作用3T3细胞后，3T3细胞出现10％的凋亡量；（2）驻极体作用3T3细胞以后，细胞质游离Ca^2 浓度升高，Ca^2 浓度的升高不是由于细胞内钙池的释放，而是由于细胞外Ca^2 内流所引起，结论：驻极体诱导3T3细胞凋亡的机制可能是细胞外Ca^2 内上起的。

Electrets induced free Ca2+ changes in 3T3 cells

Objective: To study the changes of intracellular free Ca 2+ levels in 3T3 cells induced by electrets and discuss the relationship between electrets induced apoptosis of 3T3 cells and the changes of free Ca 2+ levels. Methods: After 48 h action of -300 V and -500 V electrets in 3T3 cells, CaCl 2, A 23187 , EDTA were added in proper order to 3T3 cells. The levels of intracellular Ca 2+ and the cell number of apoptosis were detected with flow cytometry. Results:(1)After the action of electrets, 10% of 3T3 cells appeared apoptosis. (2) After the action of electrets in 3T3 cells, the free Ca 2+ levels in cellular plasm were increased. The increasement of Ca 2+ levels was caused by the Ca 2+ inflow from outside cell, rather than the release of calcium pool inside cell. Conclusion:The electret can induce Ca 2+ outside 3T3 cell to flow into cells, thereby induce cell apoptosis.