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| 海藻糖对低温保存皮肤中E钙黏素表达的影响 | |
| 引用本文： | 刘红梅,蔡宏,刘玲,杜太超. 海藻糖对低温保存皮肤中E钙黏素表达的影响[J]. 中华医学美学美容杂志, 2008, 14(3) |
| 作者姓名： | 刘红梅 蔡宏 刘玲 杜太超 |
| 作者单位： | 1. 总装备部北京黄寺美容外科医院,北京,100120 2. 解放军总医院第一附属医院 |
| 摘 要： | 目的 将非渗透性低温保护剂海藻糖用于人类皮肤的低温储存,观察海藻糖对冻存皮肤组织中E钙黏素的影响,探讨海藻糖的低温保护作用.方法 新鲜成人皮肤组织分为新鲜对照组、海藻糖-二甲基亚砜(T-D)组、二甲基亚砜-丙二醇(D-P)组均在-196 ℃液氮冻存7 d,第14天复温,免疫组织化学染色对各组间皮肤进行比较.结果 与新鲜皮片组相比,0.5 mol/L T-D能够很好保护皮肤组织,E钙黏素的蛋白表达量与新鲜皮肤组相似.结论 海藻糖与二甲基亚砜联合应用能较好地保护皮肤组织E钙黏素,其对皮肤组织的保存效果优于传统的低温保护剂. |
| 关 键 词： | 皮肤 海藻糖 E钙黏素 低温保存 |
Effect of trehalose on expression of E-cadherin in cryopreserved human skin |
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| LIU Hong-mei,CAI Hong,LIU Ling,DUI Tai-chao. Effect of trehalose on expression of E-cadherin in cryopreserved human skin[J]. Chinese Journal of Medical Aesthetics and Cosmetology, 2008, 14(3) | |
| Authors: | LIU Hong-mei CAI Hong LIU Ling DUI Tai-chao |
| Abstract: | Objective To investigate the change of E-cadherin expression in human skin after cryopreservation. Methods The skin tissue was treated with DMSO-propyleneglycol (TD group) and trehalose-DMSO (DP group), respectively, and cryopreserved in liquid nitrogen (-196 ℃) for 7 and 14 days and the changes after cryopreservation were compared with that of fresh skin (control group). Immunohistochemical staining was used to detect the expression of E-cadherin in the skin.Results Compared with the fresh skin, TD group showed good preservation of the skin, and the expression level of E-cadherin was similar to that in the control group. Conclusion E-cadherin is well preserved in the TD group. Therefore, trehalose-DMSO is an effective cryoprotectant for human skin, and much better than that of traditional low-temperature protectants. |
| Keywords: | Skin Trehalose E-cadherin Cryopreservation |
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