Production and biomedical applications of virus-like particles derived from polyomaviruses |
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| Authors: | Erik A. Teunissen Markus de RaadEnrico Mastrobattista |
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| Affiliation: | Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, University of Utrecht, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands |
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| Abstract: | ![]()
Virus-like particles (VLPs), aggregates of capsid proteins devoid of viral genetic material, show great promise in the fields of vaccine development and gene therapy. These particles spontaneously self-assemble after heterologous expression of viral structural proteins. This review will focus on the use of virus-like particles derived from polyomavirus capsid proteins. Since their first recombinant production 27 years ago these particles have been investigated for a myriad of biomedical applications. These virus-like particles are safe, easy to produce, can be loaded with a broad range of diverse cargos and can be tailored for specific delivery or epitope presentation. We will highlight the structural characteristics of polyomavirus-derived VLPs and give an overview of their applications in diagnostics, vaccine development and gene delivery. |
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| Keywords: | aa, amino acid AFFFF, asymmetrical-flow field flow fractionation APyV, avian polyomavirus BKPyV, BK virus bp, base pair ChPyV, chimpanzee polyomavirus CTL, cytotoxic T lymphocyte DC, dendritic cell DHFR, dihydrofolate reductase DIVA, differentiate infected from vaccinated animals dsDNA, double-stranded DNA DTT, dithiothreitol E. coli, Escherichia coli EDTA, ethylenediaminetetraacetic acid EGFP, enhanced green fluorescent protein EGTA, ethylene glycol tetraacetic acid ER, endoplasmic reticulum FPyV, finch polyomavirus GCV, ganciclovir GHPyV, goose hemorrhagic polyomavirus GRAS, generally recognized as safe GST, glutathione S-transferase HaPyV, hamster polyomavirus hEGF, human epidermal growth factor HPV, human papillomavirus JCPyV, JC virus LCMV, lymphocytic choriomeningitis virus LPyV, beta-lymphotropic polyomavirus MALS, multiple-angle light scattering MCC, Merkel cell carcinoma MCPyV, Merkel cell polyomavirus MPtV, murine pneumotropic virus MPyV, murine polyomavirus NLS, nuclear localization signal NP, nucleocapsid protein NTA, nitrilotriacetic acid OVA, ovalbumin PBS, phosphate buffered saline PLP, polyoma-like particle PNA, peptide nucleic acids PSA, prostate specific antigen PTX, paclitaxel PUUV, Puumala hantavirus PyV, polyomavirus S. cerevisiae, Saccharomyces cerevisiae Sf, Spodoptera frugiperda SR101, sulforhodamine 101 SV40, simian virus 40 TEM, transmission electron microscopy VLP, virus-like particle |
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