| 组蛋白脱乙酰化酶GST-HDAC1融合蛋白载体的构建及其在大肠杆菌中的表达 |
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| 引用本文： | 王桂玲,陈薇,王孝会,姜佩佳. 组蛋白脱乙酰化酶GST-HDAC1融合蛋白载体的构建及其在大肠杆菌中的表达[J]. 解剖科学进展, 2011, 17(6): 535-537 |
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| 作者姓名： | 王桂玲 陈薇 王孝会 姜佩佳 |
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| 作者单位： | 中国医科大学基础医学院细胞生物学教研室,卫生部细胞生物学重点实验室,沈阳110001 |
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| 基金项目： | 国家自然科学基金资助项目(No.30871294); 辽宁省自然科学基金资助项目(No.201102277) |
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| 摘 要： | 目的 构建GST-HDAC1融合蛋白表达载体,并在大肠埃希菌(E.coli)中诱导表达.方法以质粒pcDNA3.1- HDAC1为模板进行PCR,通过EcoR1单酶切位点将HDAC1插入p GEX-5X-1中,构建原核表达质粒pGEX-5X-1-HDAC1,并转化点coli DH5 α,通过利用载体上的BamH1酶切位...
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| 关 键 词： | GST-HDAC1融合蛋白 原核表达 |
Construction of GST-HDAC1 fusion protein vector and its expression in E.coli |
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| WANG Gui-ling,CHEN Wei,Wang Xiao-hui,JIANG Pei-jia. Construction of GST-HDAC1 fusion protein vector and its expression in E.coli[J]. Progress of Anatomical Sciences, 2011, 17(6): 535-537 |
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| Authors: | WANG Gui-ling CHEN Wei Wang Xiao-hui JIANG Pei-jia |
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| Affiliation: | WANG Gui-ling*,CHEN Wei,Wang Xiao-hui,JIANG Pei-jia(Department of Cell Biology,The Key laboratory of Cell Biology,Ministry of Public Health of China,College of Basic Medical Sciences,China Medical University,Shenyang 110001 China) |
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| Abstract: | Objective To construct GST-HDAC1 fusion protein expression vector and induce its expression in Escherichia coli().Methods The coding sequence of HDAC1 was amplified from the plasmid pcDNA3.1-HDAC1 by PCR and inserted into pGEX-5X-1 by EcoR1.The positive recombinants were identified by restriction endonuclease digestion and DNA sequencing.Then they were transformed into BL21,induced by IPTG and identified by SDS PAGE.Results The length of the forward fragment was 950bp identified by enzymes digestion and the... |
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| Keywords: | GST-HDAC1 fusion protein prokaryotic expression |
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