巨细胞病毒宫内感染状态与母婴垂直传播之间的关系

目的研究巨细胞病毒（HCMV）宫内感染状态即活动性与非活动性感染与母婴垂直传播之间的关系。方法对85例HCMV-IgM或HCMV-DNA阳性的母血标本用RT-PCR方法检测即刻早期（IE）mRNA和晚期（L）mRNA,并用PEP-PCR方法检测母血标本中分离出的胎儿细胞的HCMV-DNA。结果 85例母血标本中检测到IE-mRNA或L-mRNA的有57例,其中胎儿细胞HCMV-DNA呈阳性的有37例,宫内垂直传播率为64.91%。未检测到IE-mRNA和L-mRNA的28例样本中胎儿细胞HCMV-DNA呈阳性的有4例,宫内垂直传播率为14.29%。两组的垂直传播率经χ2检验有统计学差异（P〈0.001）。结论巨细胞病毒宫内感染处于活动期状态时,其发生母婴垂直传播的危险性显著高于非活动期感染状态,这对于采取正确的治疗措施和评估预后有重要的指导意义。

The relationship between human cytomegalovirus intrauterine infection and vertical transmission from mother to fetus

Objective：To discuss the relationship between human cytomegalovirus intrauterine infection and vertical transmission from mother to fetus. Methods：RT-PCR method was used to detect IE-mRNA and L-mRNA in 85 maternal blood samples which were HCMV-IgM positive or HCMV-DNA positive.And HCMV-DNA was analyzed by PEP-PCR method in fetal cells isolated from maternal blood. Results：57 out of 85 were detected IE-mRNA or L-mRNA positive,among which 37 samples of fetal cells were HCMV-DNA positive.The HCMV transmission rate was 64.91%.None of IE-mRNA or L-mRNA were detected in 28 samples and 4 of them were HCMV-DNA positive in fetal cells.It＇s transmission was 14.29%.There was statistical difference between the two groups （P〈0.001）. Conclusions：The risk of transmission rate was higher when they were actively infected.It has important meaning in taking correct therapy measures and predicting fetal health.