氩激光诱导色素兔脉络膜新生血管的实验研究

目的:研究氩激光诱导色素兔脉络膜新生血管(CNV)的实验条件。方法:分别对3组动物实验眼用不同功率(0.4,0.8,1W)的氩激光光凝视网膜,光凝后3,7,14,28,56及91d行光学相干断层扫描(opticalcoherencetomography,OCT)、荧光素眼底血管造影(fundusfluoresceinangiography,FFA)和吲哚菁绿血管造影(indocyaninegreenangiography,ICGA)后处死动物,取光凝区眼球壁制作标本,进行光镜和透射电镜观察。结果:第1组未见CNV生长,第2,3组光凝后7d出现CNV,28d达高峰,56d后开始减少。结论:高功率(0.8～1W)的氩激光能有效诱导色素兔CNV,是较好的动物模型。

The Experimental Study on the Model of Argon Laser-induced Choroidal Neovascularization in Rabbits

Objective: To study the experimental condition of argon laser-induced choroidal neovascularization (CNV) in pigmented rabbits. Methods: The retina of experimental eyes in 3 groups was photocoagulated by the argon laser in different powers (0.4, 0.8 and 1 W, respectively). The optical coherence tomography (OCT), fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA) were performed, then the rabbits were killed and eyes were examined for CNV by histopathology on day 3, 7, 14, 28, 56 and 91 post-photocoagulating. Results: In the first group, CNV was not found. CNV appeared on day 7 and reached the peak on day 28, then decreased on day 56 in the other two groups. Conclusion: This study demonstrated that high power argon laser (0.8~1 W) can be efficiently used to induce the CNV in the pigmented rabbits and provided a better way for the establishment of the animal model.