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Seminal Fluid and the Expression of MHC Class I Antigens in the Placenta of the Rat
Authors:AMAL KANBOUR-SHAKIR  DAVID T. ARMSTRONG  ANNE ROULEAU  HEINZ W. KUNZ  THOMAS J. GILL
Abstract:PROBLEM : To determine whether seminal fluid influences the expression of MHC class I antigens on the surface of basal trophoblast cells in the placenta of the rat. METHODS : Transfer of DA × DA embryos into a WF (allogeneic) or DA (syngeneic) recipient made pseudopregnant by hormonal treatment followed by mating with a vasectomized male (seminal fluid) or by mechanical stimulation (no seminal fluid). Antigen expression was determined by electron microscopic immunocytochemistry using the appropriate gold-labeled monoclonal antibodies. RESULTS : Seminal fluid did not affect the expression of MHC class I antigens on the surface of the basal trophoblast in either allogeneic or syngeneic matings. CONCLUSIONS : The suppression of the expression of paternal class I antigens on the surface of the basal trophoblast cells in allogeneic pregnancies most likely occurs at the genome level shortly after fertilization.
Keywords:Seminal fluid  placental MHC antigens  MHC antigen expression  embryo transfer  immunocytochemistry
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